Fig 2.

Liver regeneration studies demonstrate that Foxm1b-deficient (−/−) hepatocytes lack induction of the Foxm1b mRNA and protein. (A) RPA demonstrate significant reduction in expression of Foxm1b mRNA. Total RNA was prepared from regenerating livers from either fl/fl mice or Alb-Cre Foxm1b^−/− mice at indicated hours after PHx (numbers above panels) and was used for RPA to demonstrate that regenerating Foxm1b^−/− hepatocytes failed to induce high levels of Foxm1b mRNA. Immunohistochemical staining of regenerating liver sections (40 h after PHx) with Foxm1b antibody demonstrates that regenerating Alb-Cre Foxm1b^−/− hepatocytes (C) display undetectable nuclear staining of Foxm1b protein compared with regenerating Foxm1b fl/fl hepatocytes (B).