Fig 3.

Coexpression of Dnmt3a and DNMT3L results in stimulation of methylation at all CpG sites. Methylation at the SNRPN locus was analyzed by using bisulfite genomic sequencing on samples recovered from cells carrying pFC19 and expressing Dnmt3a alone (Left) or Dnmt3a and DNMT3L together (Right). The distribution of methylated sites (•) at each of the 23 CpG sites along the top strand of the SNRPN IC is depicted. A histogram representing the total number of methylation events observed at a given site for the whole sample is shown at the bottom. Sample size was identical for both categories (53 independent molecules analyzed per group).