Fig 2.

Basal and IFN-γ-induced activity of luciferase reporter vectors carrying the human IL-18BP promoter. Insert size, extending upstream of the transcription start site (+1), is shown in parentheses. Circled numbers represent the various response elements: 1, GAS; 2, IRF-E; 3, C/EBP-E (two sites); 4, silencer; 5, distal enhancer. Filled squares depict mutation in a specific response element. Hep G2 cells were cotransfected with the indicated reporter vector and pSV40 β-galactosidase. All luciferase values were normalized to β-galactosidase activity. (A) Luciferase activity in extracts of uninduced cells relative to that of cells transfected with pGL3-basic vector. (B) Luciferase activity in cells transfected with the indicated vectors and induced with IFN-γ. Fold induction is over basal activity as given in A.