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. 2002 Dec 16;99(26):16963–16968. doi: 10.1073/pnas.012681099

Fig 4.

Fig 4.

Soluble recombinant HLA-Cw3 and CD160 proteins bind to JAR-Cw3 and Jurkat-CD160, respectively. (A Upper) By flow cytometry, mAb CL1-R2 stained Jurkat-CD160 but not Jurkat cells (black profiles). Open profiles are Ig-isotype control stainings. (A Lower) flow cytometry binding of soluble HLA-Cw3 cross-linked with B1.23.2 mAb, followed by incubation with PE-streptavidin on Jurkat-CD160 and NK.3.3 cells but not Jurkat (black profiles). Open profiles are control staining with B1.23.2 mAb and streptavidin-PE. (B) Flow cytometry showing binding of mAb B1.23.2 on JAR-Cw3 but not JAR (Upper, open profiles are Ig-isotype control staining), and of soluble CD160-Flag, after incubation with anti-Flag mAb followed by PE-labeled conjugate on Jurkat-CD160 and NK.3.3 cells but not Jurkat (Lower, open profiles are staining after incubation with control Jurkat supernatant). (C) Cytotoxicity of NK92 cultured without IL-2 against JAR-Cw3 target cells in the absence of mAb (no mAb) or in the presence of mAb CL1-R2 (CD160). Results are mean ± SD of triplicates and are representative of three independent experiments.