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. 2006 Feb;44(2):589–591. doi: 10.1128/JCM.44.2.589-591.2006

FIG. 1.

FIG. 1.

PCR-RFLP analysis of the Cpgp40/15 locus from representative isolates. (A) Secondary PCR amplicons digested with RsaI; (B) secondary PCR amplicons digested with AluI. The first three lanes of both panels show the RFLP profiles for known subgenotype Ib, Id, and IIa Cpgp40/15 sequences previously deposited in GenBank (GenBank accession numbers AF440626, AF440625, and AF114166, respectively), while the last three lanes in each panel show the PCR-RFLP profiles from isolates 5 (a representative type Ib isolate), 7, and 15, respectively. Lane M, markers. Following RsaI digestion, the expected sizes of the PCR products, based on the known sequences of isolates with GenBank accession numbers AF440626 (type Ib), AF440625 (type Id), and AF114166 (type IIa), were 387, 200, 195, 159, and 35 bp for the Ib subtype; 483, 367, 200, and 30 bp for the Id subtype; and 329, 207, 195, 177, and 35 bp for the IIa subtype. Following AluI digestion, the expected sizes of the PCR products were 457, 380, 85, and 56 bp for the Ib subtype; 520, 385, and 175 bp for the Id subtype; and 294, 258, 214, 81, 60, and 36 bp for the IIa subtype.