TABLE 4.
Molecular identification of Zygomycetes from frozen tissues of mice experimentally infected with six different speciesa
| Species | Presence of hyphaeb (no. of samples positive/no. tested)
|
Positive PCRc (no. of samples positive/no. tested)
|
||
|---|---|---|---|---|
| Kidney | Brain | Kidney | Brain | |
| A. corymbifera | 5/6 | 2/6 | 5/6 | 2/6 |
| M. circinelloides | 2/6 | 2/6 | 5/6 | 4/6 |
| M. indicus | 4/4 | 4/4 | 4/4 | 4/4 |
| R. pusillus | 1/5 | 4/5 | 1/5 | 4/5 |
| R. microsporus | 4/5 | 2/5 | 5/5 | 4/5 |
| R. oryzae | 2/3 | 2/3 | 3/3 | 2/3 |
a
Mice were infected with 104, 105, or 106 spores per animal (1 to 2 mice per inoculum).
b
After staining of the cell wall of the fungi with Calcofluor White, the presence of hyphae was determined by direct examination of the homogenized tissue with an epifluorescence microscope.
c
All tissues containing hyphae were also positive by PCR.