TABLE 1.
log10 copies | HIV-1 RNA
|
HIV-1 DNA
|
HSV-2 DNA
|
||||||
---|---|---|---|---|---|---|---|---|---|
CV (%) of CT values | SD of CT values | SD of log10 copies | CV (%) of CT values | SD of CT values | SD of log10 copies | CV (%) of CT values | SD of CT values | SD of log10 copies | |
5 | 1.20 | 0.25 | 0.014 | 0.12 | 0.02 | 0.003 | 0.29 | 0.06 | 0.005 |
4 | 0.78 | 0.21 | 0.011 | 0.33 | 0.07 | 0.008 | 0.31 | 0.07 | 0.006 |
3 | 1.33 | 0.41 | 0.031 | 0.51 | 0.16 | 0.012 | 0.35 | 0.09 | 0.014 |
2 | 1.00 | 0.36 | 0.099 | 1.34 | 0.46 | 0.057 | 1.00 | 0.21 | 0.112 |
1 | 2.18 | 0.82 | 0.158 | 1.17 | 0.45 | 0.160 | 2.87 | 0.82 | 0.503 |
Repeatability precision is determined by the CV of CT values and standard deviation (SD) of the values obtained by 10 simultaneous determinations of serial dilutions from HIV-1 RNA culture supernatant, HIV-1 proviral DNA from 8E5 cells, and HSV-2 DNA from pcDNA3.1/HisC-ul30hsv plasmid.