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. 2006 Feb;44(2):423–432. doi: 10.1128/JCM.44.2.423-432.2006

TABLE 1.

Repeatability assay resultsa

log10 copies HIV-1 RNA
HIV-1 DNA
HSV-2 DNA
CV (%) of CT values SD of CT values SD of log10 copies CV (%) of CT values SD of CT values SD of log10 copies CV (%) of CT values SD of CT values SD of log10 copies
5 1.20 0.25 0.014 0.12 0.02 0.003 0.29 0.06 0.005
4 0.78 0.21 0.011 0.33 0.07 0.008 0.31 0.07 0.006
3 1.33 0.41 0.031 0.51 0.16 0.012 0.35 0.09 0.014
2 1.00 0.36 0.099 1.34 0.46 0.057 1.00 0.21 0.112
1 2.18 0.82 0.158 1.17 0.45 0.160 2.87 0.82 0.503
a

Repeatability precision is determined by the CV of CT values and standard deviation (SD) of the values obtained by 10 simultaneous determinations of serial dilutions from HIV-1 RNA culture supernatant, HIV-1 proviral DNA from 8E5 cells, and HSV-2 DNA from pcDNA3.1/HisC-ul30hsv plasmid.