TABLE 6.
HIV-1 RNA and HSV-2 DNA quantifications by real-time RT-PCR or real-time PCR in four pools of HIV- and HSV-negative cervicovaginal lavage samples spiked with large (250,000 copies/ml) or small (2,500 copies/ml) amounts of HIV-1 RNA and with large (100,000 copies/ml) or small (1,000 copies/ml) amounts of HSV-2 DNA
| Pool | Amount of HIV-1 RNA | Amount of HSV-2 DNA | HIV-1 RNAa
|
HSV-2 DNAa
|
||
|---|---|---|---|---|---|---|
| Expected result | Observed result | Expected result | Observed result | |||
| Pool 1 | Large | Large | 5.40 | 5.39 | 5.00 | 5.11 |
| Pool 2 | Small | Large | 3.70 | 3.89 | 5.00 | 5.00 |
| Pool 3 | Large | Small | 5.40 | 5.39 | 3.00 | 3.10 |
| Pool 4 | Small | Small | 3.70 | 3.59 | 3.00 | 2.96 |
a
Observed results are expressed as log10 copies/ml and are the mean values obtained for each pool tested in triplicate.