TABLE 1.
Sequences of primers and TaqMan probes used for detection of Beijing and non-Beijing type strains
| Primers and probe for target strains | Name | Sequence |
|---|---|---|
| Non-Beijing strainsa | ||
| Forward primer | nBjF | 5′-AAGCATTCCCTTGACAGTCGAA-3′ |
| Fluorogenic probe | nBjTM | 5′-6FAM-TCCAAGGTCTTTG-MGB-NFQ-3′c |
| Reverse primer | nBjR | 5′-GGCGCATGACTCGAAAGAAG-3′ |
| Beijing strainsb | ||
| Forward primer | BjF | 5′-CTCGGCAGCTTCCTCGAT-3′ |
| Fluorogenic probe | BjTM | 5′-YAK-AACGCCAGAGACCAGCCGCCGGCT-DB-3′d |
| Reverse primer | BjR | 5′-CGAACTCGAGGCTGCCTACTAC-3′ |
a
The primers and probe for non-Beijing strains were based on GenBank accession no. BX842581, nucleotides 7896 to 7990. The fragment generated by the primers was 95 bp in length.
b
The primers and probe for Beijing strains were based on GenBank accession no. AF390039, nucleotides 820 to 948. The fragment generated by the primers was 129 bp in length.
c
MGB-NFQ, minor groove binding, nonfluorescent quencher.
d
YAK-DB, Yakima yellow-DABCYL (nonfluorescent dark quencher).