PRSI profiles of six Leishmania strains digested with RsaI. PRSI restriction patterns of six strains were obtained after 2 h of digestion with RsaI. From left to right, the strains studied were Leishmania (Leishmania) infantum Li75, L. (L.) amazonensis La395, Leishmania (Viannia) braziliensis Lb2252, L. (V.) lainsoni Ll2208, L. (V.) naiffi Ln2204, and L. (V.) guyanensis Lg4745. A 100-bp low-molecular-size ladder (L) was used, and electrophoresis was performed for 4 h. The positions of molecular size markers (in base pairs) are shown to the right of the gel.