TABLE 2.
Fragment diversity and polymorphism in the SSU-ITS1 region
| Species | Unit size | AluI
|
ApoI
|
BfaI
|
DdeI
|
HaeIII
|
HpaII
|
PstI
|
RsaI
|
TaqI
|
Polymorphismc | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NFa | SFb | NF | SF | NF | SF | NF | SF | NF | SF | NF | SF | NF | SF | NF | SF | NF | SF | |||
| Leishmania (Leishmania) species | ||||||||||||||||||||
| L. (L.) infantum | ||||||||||||||||||||
| Genotype group I (Li75, Li307) | 1.3 | 8 | 3.1 (0.6) | 6 | 2.7 (1.2) | 0 | 0.0 | 0 | 0.0 | 7 | 2.7 | 4 | 1.9 (0.2) | 3 | 1.4 (0.4) | 7 | 3.2 (1.8) | 6 | 1.5 (0.5) | 40 |
| Genotype group II (Li417, Li1208, Li2205) | 1.3 | 7 | 2.7 (0.2) | 10 | 4.5 (0.7) | 0 | 0.0 | 0 | 0.0 | 7 | 2.7 | 4 | 1.8 | 2 | 1.5 (0.5) | 5 | 2.0 | 7 | 2.4 | |
| L. (L.) mexicana | ||||||||||||||||||||
| Lm695 | 1.3 | 7 | 2.9 | 5 | 2.4 | 0 | 0.0 | 0 | 0.0 | 7 | 3.2 | 3 | 1.3 | 3 | 2.1 | 6 | 2.6 | 4 | 0.9 | |
| Lm279 | 1.3 | 10 | 4.4 | 4 | 1.6 | 0 | 0.0 | 0 | 0.0 | 5 | 1.2 | 3 | 1.2 | 4 | 1.5 | 5 | 2.0 | 5 | 1.4 | |
| L. (L.) amazonensis | 1.3 | 9 | 4.2 (0.1) | 4 | 2.5 (0.4) | 0 | 0.0 | 0 | 0.0 | 7 | 3.0 (0.1) | 5 | 2.5 (0.4) | 3 | 1.9 (0.4) | 5 | 2.2 (0.4) | 7 | 2.2 (0.7) | 32 |
| L. (L.) aristidesi | 1.3 | 10 | 4.4 | 4 | 2.0 | 0 | 0.0 | 0 | 0.0 | 6 | 2.9 | 3 | 1.4 | 3 | 1.7 | 6 | 2.8 | 8 | 2.2 | |
| L. (L.) hertigi | 1.3 | 7 | 3.0 | 4 | 2.6 | 0 | 0.0 | 3 | 1.5 | 2 | 1.2 | 4 | 2.1 | 3 | 2.2 | 6 | 2.0 | 3 | 0.8 | |
| L. (L.) deanei | 1.3 | 6 | 2.0 | 4 | 1.7 | 0 | 0.0 | 2 | 1.3 | 2 | 1.4 | 4 | 1.9 | 2 | 1.3 | 7 | 3.4 | 8 | 2.2 | |
| Leishmania (Viannia) species | ||||||||||||||||||||
| L. (V.) braziliensis | ||||||||||||||||||||
| Genotype group I (Lb396, Lb469, Lb2222, Lb2242) | 1.2 | 11 | 4.3 (0.5) | 7 | 3.5 (0.9) | 2 | 1.3 | 3 | 1.5 (0.4) | 8 | 4.2 (0.6) | 5 | 2.3 (0.4) | 4 | 2.3 (0.2) | 6 | 3.3 (0.9) | 6 | 2.0 (0.8) | 52 |
| Lb2252 | 1.2 | 9 | 3.8 | 5 | 2.5 | 2 | 1.3 | 4 | 2.0 | 9 | 4.8 | 6 | 2.9 | 4 | 1.9 | 5 | 2.4 | 9 | 3.1 | |
| L. (V.) peruviana | 1.1 | 8 | 3.3 | 6 | 3.4 | 4 | 2.8 | 4 | 1.4 | 3 | 1.3 | 5 | 2.3 | 4 | 2.4 | 5 | 3.5 | 4 | 0.8 | |
| L. (V.) guyanensis | 1.2 | 9 | 3.3 (0.3) | 2 | 1.1 | 0 | 0.0 | 0 | 0.0 | 4 | 1.5 (0.7) | 6 | 2.4 (0.5) | 3 | 1.2 (0.1) | 2 | 0.8 (0.3) | 5 | 1.1 | 40 |
| L. (V.) naiffi | ||||||||||||||||||||
| Genotype group I (Ln2807, Ln3428) | 1.3 | 9 | 3.4 (0.6) | 7 | 3.6 (0.1) | 2 | 1.2 | 2 | 1.2 | 7 | 3.2 (1.6) | 6 | 2.7 (0.9) | 3 | 1.7 (0.8) | 7 | 3.5 (0.8) | 6 | 1.5 (0.3) | 67 |
| Ln2204 | 1.3 | 6 | 2.0 | 3 | 1.3 | 2 | 1.2 | 2 | 1.2 | 5 | 2.7 | 5 | 2.0 | 2 | 1.3 | 4 | 1.2 | 8 | 2.3 | |
| L. (V.) lainsoni | ||||||||||||||||||||
| Genotype group I (Ll2227, Ll2229) | 1.2 | 12 | 5.3 (0.1) | 2 | 1.1 | 0 | 0.0 | 0 | 0.0 | 7 | 2.4 | 5 | 2.0 (0.1) | 2 | 1.1 | 4 | 1.4 (0.3) | 6 | 1.6 (0.9) | 53 |
| Ll2208 | 1.2 | 11 | 4.5 | 3 | 1.3 | 0 | 0.0 | 0 | 0.0 | 5 | 1.7 | 3 | 1.2 | 2 | 1.3 | 3 | 1.3 | 7 | 2.2 | |
| Crithidia fasciculata | 1.0 | 8 | 3.1 | 2 | 1.2 | 0 | 0.0 | 5 | 1.7 | 6 | 3.2 | 3 | 1.2 | 5 | 2.5 | 5 | 2.8 | 3 | 0.6 | |
a
NF, average number of fragments differing from the unit size.
b
SF, sum of fragment sizes. The average and standard deviation (in parentheses) are shown for some values.
c
For each species (n > 2), polymorphism is calculated by dividing the sum of the different genotypes encountered for each enzyme by the number of strains analyzed and given as a percentage.