Fig 1.

Protracted l-methionine treatment (6.6 mmol/kg, twice daily for 15 days) induced down-regulation of reelin protein expression in the FC of WTM (A) and HRM (B). Measurements were conducted after 15 days of treatment with vehicle (VEH), l-methionine (MET), or glycine (GLY) at the doses detailed in Table 1. Each value is the mean ± SE of five mice per group. The overall ANOVA (400- and 180-kDa proteins) of vehicle, l-methionine, and glycine groups yielded P < 0.001. Bonferroni multiple comparison: *, P < 0.05 for l-methionine vs. vehicle or glycine. The open and filled bars indicate 400- and 180-kDa reelin-like immunoreactive peptides, respectively. (C) Western immunoblot of reelin and β-actin after 6% SDS/PAGE. Comparison between a vehicle- (lanes 1–3, serial dilutions of the same sample) and an l-methionine-treated WTM (lanes 4–6).