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. 2002 Dec 13;99(26):17119–17124. doi: 10.1073/pnas.252624099

Fig 3.

Fig 3.

Both wild-type Notch 3 and Notch 3R142C receptors respond to Notch ligands. HEK 293 stable cell lines expressing either the wild-type mNotch 3 (Notch 3) or the mNotch 3R142C receptor were cocultured with 3T3 cells expressing either Jagged 1 (3T3-hJagged 1, black), HEK 293 cells expressing Delta 1 (293-hDelta 1, light gray), or the respective control line (Ctrl-3T3, white; Ctrl-293, white) and analyzed for 6× RBP–luciferase reporter gene activity. Similar levels of receptor activation were observed for the mNotch 3- and mNotch 3R142C-expressing cells. All transfections were performed in triplicate, and the experiment was repeated twice.