Fig. 2.

Double-staining with a BN serum shows complete colocalization with α-MSH (a–c) and partial colocalization with CART peptide (d–f) in the arcuate nucleus (Arc). However, some CART-positive/BN-negative cells (arrowheads) are seen in this nucleus and in terminals in the median eminence (ME) (d–f). In the pituitary, AN serum stains melanotropes in the intermediate lobe (IL) and corticotropes in the anterior lobe (AL) as revealed with ACTH double-staining (g–i). Colocalization of AN serum immunostaining with LHRH in fibers in the ME (j–l). PL, posterior pituitary lobe; 3v, third ventricle. [Scale bars = 50 μm (a–c and g–l) and 100 μm (d–f).]