Fig 1.

Mating tests. After drug treatment, fertility of male mice was assessed by caging each of them with four female mice for 7 (A) or 9 (B) days, during which the females were monitored for vaginal mucous plugs (see text), and subsequently for pregnancies and litter sizes. (A) Effects of oral administration and withdrawal of NB-DNJ on fertility of male mice. ON, Animals were given the compound for 1–5 weeks. OFF, Recovery of fertility was studied by using males that had received the compound for 5 weeks. These animals were further assayed for 5 weeks without drug by caging each of them each week with a new set of four female mice. (B) Comparison of effects of imino sugars with different biochemical activities on reproductive capacity of male mice. The data are presented as the mean ± SD for 3 male and 12 female mice per data point.