Fig 2.

Dose-effect study. Males were given NB-DNJ in a range of concentrations for 5 weeks, and then caged with four female mice each for 9 days (n = 6 males in A, n = 3 males in B). The females were monitored for pregnancies and litter sizes (A) and vaginal mucous plugs (B). Alternatively, after a 5-week treatment, epididymal spermatozoa were obtained and incubated with either monoclonal antibody Mab18.6 or anti-sp56, and the nuclear dye Hoechst 33342. By examining >100 spermatozoa per mouse, we determined the percentage of nuclei with grossly abnormal morphology (n ≥ 4; C) and the percentage of spermatozoa that stain with monoclonal antibodies Mab18.6 (n = 3) or anti-sp56 (n = 2) (D). The data are presented as the mean ± SD.