Fig 4.

Histology of the testis and immunocytochemistry of epididymal spermatozoa. Sections from stage I seminiferous tubules from a control mouse (A) and a mouse treated with 50 mg/kg/day NB-DNJ (B). Insets show close-ups of step 13 spermatid heads. Epididymal spermatozoa from normal mice (C), 15 mg/kg/day NB-DNJ-treated mice (D), and mice recovered from a 15 mg/kg/day NB-DNJ regimen (E) were incubated with anti-acrosomal monoclonal Mab18.6 and counterstained with the nuclear dye propidium iodide. (Original magnification of A and B = ×1,000; bar in C–E = 10 μm.)