TABLE 1.
Bacterial strains and plasmids
| Bacterial strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| E. coli strains | ||
| VCS257 | Derivative of DP50supF [supE44 supF58 hsdS3(rB−mB−) dapD8 lacY1 glnV44 Δ(gal-uvrB)47 tyrT58 gyrA29 tonA53 Δ(thyA57)] | Stratagene |
| JM109 | recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ(lac-proAB)/F′ [traD36 proAB+lacIqlacZΔM15] | Takara Bio |
| DH5α | F− φ80d lacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK− mK+) phoA supE44 λ−thi-1 gyrA96 relA1 | Takara Bio |
| HB101 | leuB6 proA2 recA13 thi-1 ara-14 lacY1 galK2 xyl-5 mtl-1 rpsL20 supE44 Δ(mcrC-mrr) | Takara Bio |
| M15 | Nals Strs Rifs Thi− Lac− Ara+ Gal+ Mtl− F− RecA+ Uvr+ Lon+ | QIAGEN |
| DSM 4025 derivatives | ||
| DSM 4025 | K. vulgare strain | 34 |
| GOMTR1 | Rifr, spontaneous mutant of DSM 4025 | This study |
| GOMTR1SN::Km | Rifr Kmr, sndH gene disruptant of GOMTR1 | This study |
| Plasmids | ||
| pVK100 | ATCC 37156, RK2 (IncP) derivative, 23 kb, Kmr Tcr, cos | ATCCb |
| pRK2013 | RK2 (IncP) derivative, 45 kb, Kmr, mob | ATCC |
| pSUP202 | Apr Tcr Cmr, mob | 28 |
| pSUPSN | pSUP202 carrying sndH | This study |
| pSUPSN::Km | pSUP202 carrying sndH disrupted with Kmr gene cassette | This study |
| pQE30 | Six-His tag, Apr | QIAGEN |
| pUC18/19 | Apr | Takara Bio |
| pUC4K | Apr Kmr | TOYOBO |
a
Apr, ampicillin resistance; Cmr, chloramphenicol resistance; Kmr, kanamycin resistance; Tcr, tetracycline resistance; Rifr, rifampin resistance.
b
ATCC, American Type Culture Collection.