FIG. 2.

Effect of NH₄Cl as a nitrogen source on pelB expression and PL secretion during growth of C. gloeosporioides in culture medium. At different times after transfer to secondary medium buffered with phthalate buffer (pH 6.0) in the presence of 50 mM NH₄Cl (▪), the level of secreted PL was analyzed by Western blot analysis of concentrated, dialyzed culture medium, and pelB expression was analyzed in the mycelia. Growth of the fungus in the presence of 50 mM KNO₃ (□) was used as a control. (A) pelB expression by real-time PCR. (B) PL secretion in the presence of NH₄Cl. (C) PL secretion in the presence of increasing concentrations of NH₄Cl 16 h after transfer to secondary medium. Blot analyses were repeated three times, and results from a representative experiment are presented.