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. 2006 Feb;72(2):1226–1230. doi: 10.1128/AEM.72.2.1226-1230.2006

TABLE 1.

Gene-specific primers for RT-PCR and sequencing reactions

Primer DNA sequence (5′-3′) Use(s) Genomic locationa
IIIF1 TTT AGG GGG TCA CCT CAC A RT-PCR, sequencing 11-29 (Q-beta)
IIIF2 CCC GTA GGG GGG TAC TCT AT RT-PCR, sequencing 9 (MX1)
IIIR AGC CAG AGA TTA CCA GCA GTA GC RT-PCR 660 (Q-beta and MX1)
IIIRQb TGC GTG TCR GAA GAT TCG Sequencing 150 (Q-beta)
IIIRMX1 TTG TCC CAC TCA GCC CTC AT Sequencing 347 (MX1)
a

Genomic locations are based on sequences presented by Beekwilder et al. (3).