TABLE 1.
Gene-specific primers for RT-PCR and sequencing reactions
| Primer | DNA sequence (5′-3′) | Use(s) | Genomic locationa |
|---|---|---|---|
| IIIF1 | TTT AGG GGG TCA CCT CAC A | RT-PCR, sequencing | 11-29 (Q-beta) |
| IIIF2 | CCC GTA GGG GGG TAC TCT AT | RT-PCR, sequencing | 9 (MX1) |
| IIIR | AGC CAG AGA TTA CCA GCA GTA GC | RT-PCR | 660 (Q-beta and MX1) |
| IIIRQb | TGC GTG TCR GAA GAT TCG | Sequencing | 150 (Q-beta) |
| IIIRMX1 | TTG TCC CAC TCA GCC CTC AT | Sequencing | 347 (MX1) |
a
Genomic locations are based on sequences presented by Beekwilder et al. (3).