TABLE 1.

Primers

Specificity	Primer name	Sequence	PCR product size (bp)
16S rRNA; all members	EC9	5′-TACCTTGTTACGACTT>	1,462
	EC12A	5′-TGATCCTGGCTCAGAACGAACG
16S rRNA E. muris	EM87f	5′-GGTTCGCTATTAGTGGCAGA	517
	EM584r	5′-CAGTATTAAAAGCCGCTCCA
16S rRNA Ehrlichia HF strain	HF51f	5′-AAGTCGAACGGACAATTACC	923
	HF954r	5′-GTTAGGGGGATACGACCTTC
16S rRNA N. mikurensis	IS58-62f	5′-GGAATAGCTGTTAGAAATGACA	488
	IS58-594r	5′-CTATCCTCTCTCGATCTCTAGTTT
16S rRNA A. phagocytophilum	SSAP2f	5′-GCTGAATGTGGGGATAATTTAT	641
	SSAP2r	5′-ATGGCTGCTTCCTTTCGGTTA
16S rRNA A. centrale	AC1f	5′-CTGCTTTTAATACTGCAGGACTA	426
	AC1r	5′-ATGCAGCACCTGTGTGAGGT
16S rRNA A. bovis	AB1f	5′-CTCGTAGCTTGCTATGAGAAC	551
	AB1r	5′-TCTCCCGGACTCCAGTCTG
p44	p3709	5′-GCTAAGGAGTTAGCTTATGA	∼400
	p4257	5′-AGAAGATCATAACAAGCATTG
p44	p3761	5′-CTGCTCTKGCCAARACCTC	∼300
	p4183	5′-CAATAGTYTTAGCTAGTAACC