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. 2006 Feb;72(2):1027–1033. doi: 10.1128/AEM.72.2.1027-1033.2006

TABLE 2.

Panel of group- and species-specific 16S rRNA-targeted oligonucleotide probes

Target Probe Sequence (5′ to 3′)c OPD codea Reference
Domain Bacteria EUB 338 GCTGCCTCCCGTAGGAGT S-D-Bact-0338-a-A-18 1
NON 338 ACATCCTACGGGAGGC NAb 45
Bacteroides-Prevotella Bac 303 CCAATGTGGGGGACCTT S-*-Bacto-0303-a-A-17 28
Bacteroides putredinis Bputre 698 GTTCTGTATGATCTCTAAGC S-S-Bputr-0698-a-A-20 37
Bacteroides vulgatus Bvulg 1017 AGATGCCTTGCGGCTTACGGC S-S-Bvulg-1017-a-A-21 37
Enterobacteria Enter 1432 CTTTTGCAACCCACT S-*-Ent-1432-a-A-15 41
Lactobacillus-Streptococcus group Lab 158 GGTATTAGCAYCTGTTTCCA S-G-Lab-0158-a-A-20 14
Bifidobacterium group Bif 164 CATCCGGCATTACCACCC S-G-Bif-0164-a-A-18 25
Atopobium cluster Ato 291 GGTCGGTCTCTCAACCC S-*-Ato-0291-a-A-17 16
Clostridium coccoides-Eubacterium rectale cluster Erec 482 GCTTCTTAGTCARGTACCG S-*-Erec-0482-a-A-19 12
Faecalibacterium prausnitzii cluster Fprau 645 CCTCTGCACTACTCAAGAAAAAC S-*-Fprau-0645-a-A-23 43
Ruminococcus group Rbro 730 TAAAGCCCAGYAGGCCGC S-*-Rbro-0730-a-A-18 15
cpRbro1d TAAAGCCCAGYAAGCCGC 26
cpRbro2d GGTGCCCAGYAGGCCGC 26
Clostridium viride group Cvir 1414 GGGTGTTCCCGRCTCTCA S-*-Cvir-1414-a-A-18 26
cpCvir1d GGGTGTTDCCGRCTCTCA 26
cpCvir2d GGGTGTTCCCGRCTTTCA 26
cpCvir3d GGGTCCCCCCGRCTCTCA 26
Eubacterium cylindroides subgroup Ecyl 387 CGCGGCATTGCTCGTTCA S-*-Ecyl-0387-a-A-18 15
Streptococcus-Lactococcus group Strc 493 GTTAGCCGTCCCTTTCTGG S-*-Strc-0493-a-A-19 12
cpStrcd TTTAGCCGTCCCTTTCTGG 26
Clostridium ramosum assemblage Cra 757 CCACGCTTTCGKGAMTGA S-S-Cra-0757-a-A-18 26
cpCrad CCACGCTTTCGKGAMTCA 26
a

OPD code, oligonucleotide probe database code.

b

NA, not applicable.

c

Sequence in IUPAC code: R = G/A, Y = T/C, M = A/C, K = G/T, S = G/C, W = A/T, H = A/C/T, B = G/T/C, V = G/C/A, D = G/A/T, N = G/A/T/C.

d

Competitor oligonucleotide probe.