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. 2006 Feb;72(2):1302–1309. doi: 10.1128/AEM.72.2.1302-1309.2006

FIG. 1.

FIG. 1.

(a) RT-PCR analysis of styE mRNA expression in P. putida CA-3 grown on various carbon sources. Lanes: 1, succinate; 2, styrene; 3, phenylacetic acid; 4, P. putida CA-3 genomic DNA as positive control; M, molecular weight markers. (b) RT-PCR analysis of citrate synthase mRNA expression in P. putida CA-3. Lanes: 1, succinate; 2, styrene; 3, phenylacetic acid as a sole carbon source; 4, P. putida CA-3 genomic DNA as a positive control; M, molecular weight markers. (c) RT-PCR analysis of total RNA from a styrene-grown culture of P. putida CA-3, assessing operonic expression of styE. Lanes: 1, 1,508-bp product obtained with styDF/styDR; 2, black arrow indicating 1,730-bp product obtained with styDF(1074)/styER; 3, 540-bp product obtained with CitF/CitR; M, molecular weight markers.