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. 2006 Feb;72(2):1588–1594. doi: 10.1128/AEM.72.2.1588-1594.2006

FIG. 1.

FIG. 1.

Comparison of d-amino acid aminotransferases from thermophilic Bacilli strains using Western blotting analyses. Lanes 1 to 7 are the immunostains for the cell extracts from different Bacillus strains: 1, YM1; 2, LK1; 3, LK2; 4, SK1; 5, KLS1; 6, KL1; and 7, SD1. The polyclonal antibody was prepared from an antiserum boosted by the YM1 d-AAT. Lanes 8 to 11 are the purified d-AATs: 8 and 10, Bacillus sp. strain YM1; 9 and 11, G. toebii SK1. Lanes 10 and 11 are the sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the purified YM1 and SK1 proteins.