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Kinetic parameters of d-AATs
| Enzyme | wXXcXik | Mean ± SD
|
kcat/Km,d-Ala (mM · s)−1 | kcat/Km,α-KG (mM · s)−1 | ||
|---|---|---|---|---|---|---|
| kcat (s−1) | Km,d-Ala (mM) | Km,α-KG (mM) | ||||
| YM1 | 140-LRcD-143 | 134 ± 2 | 2.5 ± 0.2 | 3.1 ± 0.1 | 52 | 42 |
| SK1 | 144-EYcY-147 | 47 ± 2 | 2.5 ± 0.1 | 0.71 ± 0.1 | 18 | 63 |
| KLS1 | 144-EYcY-147 | 55 ± 3 | 1.4 ± 0.2 | 0.26 ± 0.1 | 38 | 203 |
| Mutant SK1 | 144-LRcD-147 | 79 ± 6 | 3.4 ± 0.3 | 1.4 ± 0.3 | 22 | 53 |
