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. 2002 Dec 16;99(26):17197–17202. doi: 10.1073/pnas.212648799

Fig 1.

Fig 1.

Western blots showing levels of Hb proteins in transgenic and WT (C24) plants. (A) GLB1 in shoot or root tissues from control plants or plants subjected to 5% or 0.1% oxygen. Lanes contain protein extracted from shoot tissue control (SC), shoot 5% oxygen (S5), shoot 0.1% oxygen (S0.1), root tissue control (RC), root 5% oxygen (L5), and root 0.1% oxygen (R0.1) plants. GLB1 bands migrated at an apparent size of 18 kDa. (B) GLB1 overexpression in the 35S:GLB1 plant lines D1, D4, and D11 compared with WT control (the picture has been split to remove lanes). (C) GLB1 overexpression in the 35S:GLB1 plant lines D4, D5, and D7 and in plant lines overexpressing GLB1(HE7L) (lines E74, E75, and E76) are shown compared with WT. (D) P. andersonii GLB1S levels for 35S:GLB1S(Pa4) and 35S:GLB1S(Pa5) plants and WT controls. High levels of P. andersonii GLB1S were produced in the transgenic plants, but these levels cannot be compared with the levels of A. thaliana GLB1 shown in A–C, because these Western blots use different antisera.