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. 2006 Feb;72(2):1096–1101. doi: 10.1128/AEM.72.2.1096-1101.2006

FIG. 2.

FIG. 2.

Preparation of aflX knockout mutants. (A) Schematic diagram of the knockout vector used to prepare the aflX knockout mutants. The dashed lines show the results expected by replacement of the wild-type DNA with DNA containing the niaD transformant selection cassette. Direction of transcription is indicated by horizontal arrows. The lengths of expected DNA fragments upon restriction enzyme digestion of either the wild-type A. flavus or the transformant DNA are shown under the horizontal lines. (B) TLC profiles of putative aflX knockout and nonknockout niaD+ transformants produced by transformation of A. flavus AF13 with the aflX knockout cassette shown in panel A. For TLC, 10 μl of culture medium was spotted on silica plates and developed with toluene-ethyl acetate-acetic acid (80:10:10 [vol/vol/vol]). The position of the aflatoxin B1 (AFB1) standard is shown on the right. Lanes are designations of individual transformant colonies. The plates were visualized under UV light at 312 nm.