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. 2006 Feb;72(2):1096–1101. doi: 10.1128/AEM.72.2.1096-1101.2006

FIG. 3.

FIG. 3.

Results of Southern blot and RT-PCR analyses. (A) Southern blot results. Lanes: 1, AF13, XhoI; 2, Transformant O1, XhoI; 3, AF13, EcoRI; 4, O1, EcoRI; 5, AF13, HindIII; 6, O1, HindIII. (B) RT-PCR results. RT was carried out on total RNA digested with RNase-free DNase I with oligonucleotide primers specific for the coding regions of aflX and the flanking genes aflY and moxY. Lanes: 1, AF13 genomic DNA control; 2, AF13 cDNA; 3, aflX knockout transformant O1 cDNA; 4, no template negative control.