TABLE 1.
Expt (precursor feeding)a | AF produced (ng/5-ml culture)b |
---|---|
None | 220 |
AVN | 210 |
AVF | 280 |
HAVN | 270 |
VA | 240 |
ST | 620 |
OMST | 750 |
Abbreviations: AVN, averantin; AVF, averufin; HAVN, hydroxyaverantin; ST, sterigmatocystin; OMST, O-methylsterigmatocystin.
Quantification of AF was by comparison of fluorescence on silica gel TLC plates to that of known amounts of authentic standards. The limit of detection was 5 ng. Experiments were repeated twice with similar results.