TABLE 1.

Database typing of ITS sequences^a

Sum (%)b	OTU	Accession no.	Best BLAST hit					% Similaritye
			Taxon	Phylum	Class	Scorec	Accession no.d
10.58	Ms9Mb4f	AJ875353	Cladosporium sp. strain 4/97-17	Ascomycota	Dothideomycetes	1,158	AJ279487	99.8
5.27	Ms4Mb4af	AJ875341	Unidentified isolate 4/97-9	Ascomycota	Sordariomycetes	1,088	AJ279484	100
5.11	Ms6Mb4	AJ875345	Chaetomium globosum	Ascomycota	Sordariomycetes	476	AY429056	80.5
3.70	Ms28Mb4a	AJ875376	Cercophora appalachianensis	Ascomycota	Sordariomycetes	667	AF177155	79.6
2.55	Ms9Mb43a	AJ875357	Stenella araguata	Ascomycota	Dothideomycetes	359	AF362066	81.0
2.45	Ms39Mb28g	AJ875381	Aureobasidium pullulans	Ascomycota	Dothideomycetes	1,197	AF455533	99.7
2.45	Ms51Mb4	AJ875391	Thielavia intermedia	Ascomycota	Sordariomycetes	603	AJ271588	89.9
2.40	Ms9Mb43b	AJ875358	Cladosporium sp.	Ascomycota	Dothideomycetes	581	AJ222807	83.3
2.35	Ms22Mb7	AJ875369	Ericoid mycorrhiza	Ascomycota	Leotiomycetes	928	AY046400	98.0
2.35	Ms25Mb17	AJ875374	Terfezia leptoderma	Ascomycota	Pezizomycetes	509	AF396863	86.4
2.19	Ms7Mb28bf	AJ875351	Stagonospora sp. strain 4/99-18	Ascomycota	Dothideomycetes	1,185	AJ496627	100
1.82	Ms5Mb4	AJ875343	Podospora ellisiana	Ascomycota	Sordariomycetes	458	AY515360	79.6
1.77	Ms7Mb4	AJ875347	Podospora decidua	Ascomycota	Sordariomycetes	684	AF443851	90.6
1.51	Ms41Mb20	AJ875382	Hymenogaster griseus	Basidiomycota	Homobasidiomycetes	1,203	AF325636	97.8
1.46	Ms5Mb7g	AJ875344	Cryptococcus carnescens	Basidiomycota	Heterobasidiomycetes	914	AB105438	97.8
1.41	Ms20Mb11f	AJ875368	Cylindrocarpon sp.	Ascomycota	Sordariomycetes	963	AY295332	99.8
1.36	Ms25Mb24	AJ875375	Sebacina sp.	Basidiomycota	Heterobasidiomycetes	452	AF284135	88.4
1.36	Ms4Mb4b	AJ875342	Colletotrichum sublineolum	Ascomycota	Sordariomycetes	325	AJ301978	74.2
1.20	Ms48Mb21	AJ875390	Sporobolomyces roseus	Basidiomycota	Urediniomycetes	1,191	AY015438	99.8
1.09	Ms28Mb4b	AJ875377	Podospora intestinacea	Ascomycota	Sordariomycetes	549	AY515363	87.5
1.09	Ms9Mb28	AJ875356	Dactylaria junci	Ascomycota	Orbiliomycetes	379	AY265321	73.3
1.04	Ms17Mb11f	AJ875366	Cylindrocarpon sp. strain 5/97-12	Ascomycota	Sordariomycetes	1,142	AJ279482	99.7
0.99	Ms13Mb4	AJ875362	Choiromyces aboriginum	Ascomycota	Pezizomycetes	1,067	AF501259	100
0.89	Ms9Mb7	AJ875354	Cistella grevillei	Ascomycota	Leotiomycetes	702	U57089	90.2
0.78	Ms24Mb18	AJ875372	Tuber maculatum	Ascomycota	Pezizomycetes	1,215	AF106889	99.2
0.63	Ms2Mb64	AJ875339	Bullera sp.	Basidiomycota	Heterobasidiomycetes	145	AY313032	31.6
0.57	Ms13Mb9	AJ875363	Cladosporium sp.	Ascomycota	Dothideomycetes	347	AJ222807	75.9
0.57	Ms15Mb7g	AJ875364	Uncultured Phialophora	Ascomycota	Sordariomycetes	793	AY578279	98.6
0.57	Ms23Mb25	AJ875371	Kabatiella caulivora	Ascomycota	Dothideomycetes	351	AJ244251	68.0
0.57	Ms7Mb28a	AJ875350	Phaeosphaeria eustoma	Ascomycota	Dothideomycetes	1,092	AJ496629	98.0
0.57	Ms9Mb43c	AJ875359	Cladosporium sp.	Ascomycota	Dothideomycetes	381	AJ222807	79.0
0.52	Ms25Mb5	AJ875373	Calyptrozyma arxii	Ascomycota	Eurotiomycetes	244	AJ133432	60.7
0.52	Ms28Mb91	AJ875378	Sporobolomyces gracilis	Basidiomycota	Urediniomycetes	1,183	AF444578	100
0.52	Ms39Mb5	AJ875380	Stachybotrys elegans	Ascomycota	Sordariomycetes	1,207	AF081481	100
0.52	Ms8Mb20	AJ875352	Hebeloma collariatum	Basidiomycota	Homobasidiomycetes	1,259	AY309962	98.7
0.47	Ms43Mb21g	AJ875386	Unidentified ericoid mycorrhiza	Ascomycota	Undefined	307	AF149079	72.0
0.47	Ms7Mb5	AJ875348	Rhizopus sexualis	Zygomycota	Mucorales	137	AB113011	54.8
0.42	Ms43Mb9	AJ875384	Scuellinia scutellata	Ascomycota	Pezizomycetes	618	AF072091	91.1
0.36	Ms88Mb20	AJ875397	Agrocybe erebia	Basidiomycota	Homobasidiomycetes	1,209	AY168831	97.9
0.36	Ms9Mb18	AJ875355	Stenella araguata	Ascomycota	Dothideomycetes	369	AF362066	70.0
0.26	Ms43Mb11	AJ875385	Cylindrocarpon sp.	Ascomycota	Sordariomycetes	597	AY295335	89.3
0.26	Ms45Mb46	AJ875389	Cryptococcus chernovii	Basidiomycota	Heterobasidiomycetes	1,233	AF444354	99.4
0.26	Ms63Mb4	AJ875393	Phaeoacremonium inflatipes	Ascomycota	Sordariomycetes	331	AF118140	71.0
0.26	Ms88Mb26	AJ875398	Thelephoraceae sp.	Basidiomycota	Homobasidiomycetes	866	U83471	91.1
0.21	Ms9Mb105	AJ875360	Dactylaria junci	Ascomycota	Orbiliomycetes	626	AY265320	89.2
0.16	Ms20Mb5	AJ875367	Dioszegia crocea	Basidiomycota	Heterobasidiomycetes	646	AF444406	91.8
0.16	Ms44Mb20	AJ875387	Hebeloma pusillum	Basidiomycota	Homobasidiomycetes	1,181	AF124702	99.3
0.16	Ms44Mb67	AJ875388	Psathyrella gracilis	Basidiomycota	Homobasidiomycetes	1,031	AY228352	92.8
0.16	Ms56Mb4	AJ875392	Unidentified isolate	Ascomycota	Undefined	781	AY559361	96.0
0.16	Ms88Mb6	AJ875395	Uncultured ectomycorrhiza	Basidiomycota	Homobasidiomycetes	414	AY310829	79.8
0.10	Ms30Mb28	AJ875379	Phaeococcomyces nigricans	Ascomycota	Chaetothyriomycetes	337	AF050278	71.1
0.10	Ms3Mb3	AJ875340	Glomus sp.	Glomeromycota	Glomeromycetes	944	AY174689	97.7
0.10	Ms6Mb21	AJ875346	Phaeoacremonium aleophilum	Ascomycota	Sordariomycetes	359	AY159787	71.3
0.10	Ms77Mb41	AJ875394	Candida castellii	Ascomycota	Saccharomycetes	218	AY046196	44.2
0.10	Ms88Mb19	AJ875396	Sebacina vermifera	Basidiomycota	Heterobasidiomycetes	337	AF202728	73.9
0.05	Ms10Mb19	AJ875361	Uncinula septata	Ascomycota	Leotiomycetes	163	AB022421	56.5
0.05	Ms16Mb14	AJ875365	Exophiala salmonis	Ascomycota	Chaetothyriomycetes	955	AF050274	91.4
0.05	Ms1Mb17	AJ875338	Phialophora sp.	Ascomycota	Sordariomycetes	511	U31845	86.0
0.05	Ms23Mb21	AJ875370	Phaeoacremonium aleophilum	Ascomycota	Sordariomycetes	373	AY159787	71.5
0.05	Ms41Mb91	AJ875383	Agrocybe praecox	Basidiomycota	Homobasidiomycetes	1,156	AF124713	98.4
0.05	Ms7Mb20	AJ875349	Sporobolomyces yunnanensis	Basidiomycota	Urediniomycetes	1,164	AB030353	99.3

^aOTUs were characterized by MspI and MboI digestion. The suffixes a, b, and c indicate cases in which a third enzyme, PalI, was used for further differentiation.

^bRelative abundance for the combined libraries, which was used to sort the entries.

^cBLASTN score.

^dAccession number of the closest database match.

^eLevel of similarity for pairwise alignments with the closest match, using the Martinez-Needleman-Wunsch algorithm.

^fThe OTU is >97% similar to a fungal isolate that was recovered previously from reeds.

^gRelated sequences diverge up to 3%. The sequence most similar to the closest database hit was used in the analysis.