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. 2006 Feb;72(2):986–993. doi: 10.1128/AEM.72.2.986-993.2006

TABLE 1.

Oligonucleotide primersa

Primer Nucleotide sequence Expt
Oligo62 CACACTCTGATCTGGCACAGTC XYL-2 RT-PCR
Oligo65 GTAGTTGCTGTCGAACAACAGA XYL-2 RT-PCR
Oligo100 CTCATGCGCGGTAGCTTCAAGTCG Ces1 RT-PCR
Oligo167 CACAAATTTACACCGTCGCGGAC Ces1 RT-PCR
Oligo102 GCATGAGCTTGCCGTTGGCCGTG XYL-6 RT-PCR
Oligo168 GGTGCCAAGACTTGCGTCTCTGG XYL-6 RT-PCR
Oligo169 CTGAACGTTGATGTTGCTGTTTCC XYL-1 RT-PCR
Oligo170 CACGTACTCGGGCACCTTTAACC XYL-1 RT-PCR
Oligo103 pTCGACATGb pX6Sur construction
Oligo116 CACCCAACCTATCACACACC Mutant screening
Oligo117 CGATTGACGAGTTGTATTGCC Mutant screening
Oligo350 pCTAGGGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCG pPicH construction
Oligo351 pGGCCGCTACTTACTCAATGATGATGATGATGATGGTCGACGGCGC pPicH construction
Oligo352 pTCGACCATCATCATCATCATCATTGAGTAAGTAGC pPicH construction
Oligo227 TTTACGTAACCACCATGCGTACTCCCGC pHX6 cloning
Oligo359 TTTCCTAGGCAAAGCGTTCATGATGGCGGTG pHX6 cloning
a

All primers were synthesized by the Integrated Biotechnology Laboratories of the University of Georgia.

b

The italic lowercase p preceding the nucleotide sequence represents a 5′ phosphorylation.