TABLE 1.
Primer | Nucleotide sequence | Expt |
---|---|---|
Oligo62 | CACACTCTGATCTGGCACAGTC | XYL-2 RT-PCR |
Oligo65 | GTAGTTGCTGTCGAACAACAGA | XYL-2 RT-PCR |
Oligo100 | CTCATGCGCGGTAGCTTCAAGTCG | Ces1 RT-PCR |
Oligo167 | CACAAATTTACACCGTCGCGGAC | Ces1 RT-PCR |
Oligo102 | GCATGAGCTTGCCGTTGGCCGTG | XYL-6 RT-PCR |
Oligo168 | GGTGCCAAGACTTGCGTCTCTGG | XYL-6 RT-PCR |
Oligo169 | CTGAACGTTGATGTTGCTGTTTCC | XYL-1 RT-PCR |
Oligo170 | CACGTACTCGGGCACCTTTAACC | XYL-1 RT-PCR |
Oligo103 | pTCGACATGb | pX6Sur construction |
Oligo116 | CACCCAACCTATCACACACC | Mutant screening |
Oligo117 | CGATTGACGAGTTGTATTGCC | Mutant screening |
Oligo350 | pCTAGGGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCG | pPicH construction |
Oligo351 | pGGCCGCTACTTACTCAATGATGATGATGATGATGGTCGACGGCGC | pPicH construction |
Oligo352 | pTCGACCATCATCATCATCATCATTGAGTAAGTAGC | pPicH construction |
Oligo227 | TTTACGTAACCACCATGCGTACTCCCGC | pHX6 cloning |
Oligo359 | TTTCCTAGGCAAAGCGTTCATGATGGCGGTG | pHX6 cloning |
All primers were synthesized by the Integrated Biotechnology Laboratories of the University of Georgia.
The italic lowercase p preceding the nucleotide sequence represents a 5′ phosphorylation.