TABLE 2.
Putative functions of M. grisea ESTs
| Type and sequence | Accession no. | Putative functiona | Matchb |
|---|---|---|---|
| Cell wall-degrading enzymes and sugar transportation | |||
| RCW5 | AA415060 | α-l-Arabinofuranosidase (Afs1) | AL021411 |
| RCW20 [2]c | AA415086 | β-Glucosidase (EC 3.2.1.21) | S52771 |
| RCW22 | L37530 | endo-β-1,4-Xylanase (EC 3.2.1.8) (XYL-2) | L37530 |
| RCW105d | AA415141 | Group 10 glycohydrolase (XYL-6) | P46239 |
| RCW109 | L81126 | endo-β-1,4-Xylanase (EC 3.2.1.8) (XYL-3) | L81126 |
| RCW18 | AA415084 | Sugar transporter protein (STP1) | P18631 |
| RCW69 | AA415111 | Sugar transporter protein (STP2) | P108708 |
| RCW79 | AA415117 | Sugar transporter protein (STP3) | U64903 |
| Filamentous fungus-specific extracellular proteins | |||
| RCW8 [4] | AA415063 | Unknown | BI190440 |
| RCW58 | AA415101 | Phytotoxin | AL513410 |
| RCW59 | AA415102 | Unknown | BI189031 |
| Development | |||
| RCW100 | AA415137 | Sporulation-specific protein SPS2 (Ces1) | S54039 |
| RCW108 | AA415143 | Yeast meiosis-specific SPO14 (phospholipase D) | P36126 |
| Metabolism | |||
| RCW6 | AA415061 | NADP-dependent mannitol dehydrogenase | AF387300 |
| RCW7 | AA415062 | tRNA-guanine transglycosylase (EC 2.4.2.29) | P44594 |
| RCW11 | AA415064 | Eukaryotic initiation factor 4A | Z74132 |
| RCW19 [8] | AA415085 | Ergosterol synthase (C-4 sterol methyl oxidase) | U31885 |
| RCW26 | AA415091 | Triacylglycerol lipase (EC 3.1.1.3) | S49236 |
| RCW35 | AA415071 | Ubiquinol-cytochrome c reductase | P07056 |
| RCW37 | AA415072 | Stearoyl-CoA desaturase (EC 1.14.99.5) | S52745 |
| RCW50 | AA415081 | 26S protease regulator subunit 8 (factor SUGI) | S24016 |
| RCW55 | AA415099 | Nuclear control of ATPase mRNA expression (NCA3) | D63817 |
| RCW65 [6] | AA415108 | NAD-dependent formate dehydrogenase (EC 1.2.1.2) | Q07103 |
| RCW88 | AA415125 | Aspartate aminotransferase (EC 2.6.1.1) | P23542 |
| RCW91 | AA415128 | Multicatalytic endopeptidase complex, subunit PRE2 | S43739 |
| RCW92 | AA415129 | d-2-Hydroxy acid dehydrogenase (EC 1.1.99.6) | P30799 |
| RCW112 | AA415145 | Mitochondial +H transporting ATP synthase (EC 3.6.1.34) | S28794 |
| Housekeeping | |||
| RCW16 | AA415082 | 30-kDa heat shock protein 1 | P19752 |
| RCW25 | AA415090 | Clathrin assembly protein | U44890 |
| RCW110 | AA415144 | Flavohemoprotein | P39662 |
| RCW94 | AA415131 | 60S ribosomal protein LI 6 | Q10157 |
| RCW96 | AA415133 | Frequency clock protein | S44457 |
| RCW101 | AA415138 | Histone H3 | P07041 |
| RCW60 | AA415103 | Cross-pathway control protein 1 | A30208 |
| RCW68 | AA415110 | 60S ribosomal protein LI 7 | P04451 |
| RCW82 | AA415120 | 30-kDa heat shock protein 2 | P40920 |
| RCW83 [2] | AA415121 | Ubiquitin precursor | X13140 |
| Hypothetical | |||
| RCW12 [2] | AA415065 | Schizosaccharomyces pombe 35.9-kDa hypothetical protein | Q10256 |
| RCW23 | AA415088 | Probable membrane protein (Neurospora crassa) | BF072630 |
| RCW49 | AA415073 | Erysiphe graminis gEghL-6 clone homolog | L40637 |
| RCW54 | AA415098 | Emericella nidulans hypothetical protein homolog | M59935 |
| RCW63 | AA415106 | Schizosaccharomyces pombe hypothetical 8.2-kDa protein | Z69240 |
| RCW102 | AA415139 | Yeast hypothetical protein (AMDI 5′ region) | S49741 |
a
BLAST similarity search against GenBank databases (1). EC numbers and encoding genes are listed in parentheses.
b
Accession number of a matched entry with the highest probability or smallest E value (1).
c
Numbers in brackets indicate the number of redundancies in the EST pool.
d
Subject of this paper; its corresponding genomic sequence, XYL-6, has been deposited in GenBank (AY124591).