Quantification of biofilms (as described in Materials and Methods) formed by Legionella spp. on surfaces of glass (A), polystyrene (B), and polypropylene (C) at incubation temperatures of 25°C (black bars), 37°C (white bars), and 42°C (gray bars). Strains or species are indicated as strain numbers and presented in Table 1. Values are presented as a means ± standard deviation (SD) of three to six independent experiments. Statistical analysis was performed using Student's t test to compare the differences between groups, and P values of <0.05 were considered statistically significant.