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Growth of S. morelense S-30.7.5 on AF and formation of AFR. A culture of 50 ml grown on d-glucose was used to inoculate 1.5 liter of mineral medium containing 10 mM AF in a 2-liter bioreactor. The culture was grown at 28°C with aeration (4-liter air/min) and agitation (500 rpm). Growth (•), AF concentration (○), and specific AFR activity (U/mg of protein) in cell extracts (⧫) were determined. All values represent means of double determinations.
