FIG. 4.

Transcript analyses of the aurofusarin gene cluster. (A) RNA gel blots of Z03643 and its ΔGIP2 strain Tzg2-1, probed with each cluster gene. Z03643 and ΔGIP2, total RNAs from strains Z03643 and Tzg2-1 grown on carrot medium, respectively. The probe and the incubation time are indicated on the left and above, respectively. The probes used for hybridization were amplified from genomic DNA of Z03643 by use of the following primer pairs: P12-for and P12-rev for PKS12, G1-for and G1-rev for GIP1, G2-for and G2-rev for GIP2, G3-for and G3-rev for GIP3, G4-for and G4-rev for GIP4, G5-for and G5-rev for GIP5, G6-for and G6-rev for GIP6, G7-for and G7-rev for GIP7, G8-for and G8-rev for GIP8, G9-for and G9-rev for GIP9, and G10-for and G10-rev for GIP10 (Table 1). (B) RNA gel blots of Z03643 grown on carrot medium, probed with GIP2 and PKS12, respectively. The incubation times for vegetative growth and perithecia induction are indicated above the blots. The ethidium bromide-stained rRNAs are indicated as a loading control.