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. 2006 Feb;72(2):1645–1652. doi: 10.1128/AEM.72.2.1645-1652.2006

TABLE 1.

Primers used in this study

Primer Sequencea (5′ to 3′) Positionb (bp at contig) or description
G2-5′f TTTGCGGGATGATATGACTGAG 123,112-123,091 at 1.116
G2-5′r CTCCACTAGCTCCAGCCAAGC 121,978-121,999 at 1.116
AAACGCAGCTAACAGAGGAGA
G2-3′f TGAAAATTCCGTCACCAGCCTC 120,415-120,394 at 1.116
TCGCGGCAGCTTGTAATCAT
G2-3′r GCCATGCTAGCCCAACTCTCC 118,949-118,969 at 1.116
NG2-5′f GCTTGCATCGGACCGAAGGAATGA 122,880-122,856 at 1.116
NG2-3′r GCCCTGAGCTTGCAGCAGAGTGTCTT 119,164-119,189 at 1.116
P12-for TATAGGGGATTGTGCTTC 136,498-136,481 at 1.116
P12-rev AATACACAAACAGCCCTCTC 129,906-129,925 at 1.116
G1-for AGGAGCCCTCCAAGTAAT 144,157-144,140 at 1.116
G1-rev TCAACGCAGTCGATTGTA 141,752-141,769 at 1.116
G2-for ACGACCTTCCTAAACTGCACCTATCA 122,182-122,157 at 1.116
G2-rev GGATGCCTGCACCCACCTA 120,235-120,253 at 1.116
G3-for GGCTTAACGGCTGAGGACCAAT 122,940-122,961 at 1.116
G3-rev GCACGGCCGATCTCATCAAG 124,669-124,650 at 1.116
G4-for GCATCACCATTCAATCCT 127,093-127,076 at 1.116
G4-rev CCTTTTTATTGCATTGCA 125,277-125,294 at 1.116
G5-for TCTCGTCAATACCAACCC 127,538-127,555 at 1.116
G5-rev GCAGATTGCCTTCATTCT 129,844-129,827 at 1.116
G6-for CGCCTCATAGTGATACCCAAGAAA 139,925-136,948 at 1.116
G6-rev AAACGCGGATCTGCCTTCAT 137,264-137,245 at 1.116
G7-for GAACACGGCATCCACTGTAAGAT 139,307-139,285 at 1.116
G7-rev GCGATATCAGCGAGATCAAAAATA 137,647-137,670 at 1.116
G8-for CACCCCGACCCGAAGAGC 139,452-139,469 at 1.116
G8-rev CTCACAACAGTCAATCAGGAACCAC 141,641-141,617 at 1.116
G9-for TCATCAATGTCAGCCAAG 144,377-144,394 at 1.116
G9-rev CGAGTCGCACTGAGTATG 145,843-145,826 at 1.116
G10-for GCTTGCCATCTCGAGTTTGAAT 148,254-148,233 at 1.116
G10-rev CAGTGCGACTCGAATGAGGC 145,832-145,851 at 1.116
G2ov-p1 CATTACATACAGCCGTCAATA 121,713-121,692 at 1.116
..........................................................
TGAGTTCCACAGACCCCCTTC
G2ov-p2 GTGTTGGTGCCGTTGCCC 119,150-119,167 at 1.116
G2ov-p3 TGTTCGTCGTCGGCTTCGTTC 123,273-123,293 at 1.116
GCGGGGTCTACAATAAGC
G2ov-p4 GCACGGCCGATCTCATCAAG 124,669-124,650 at 1.116
bpro-5′ CGAAGCCGACGACGAACA 49,310-49,327 at 1.393    
bpro-3′ ATTGACGGCTGTAGATGTAATG 51,455-51,434 at 1.393    
.................................................................
NG2ov-5′f ACCCAATACTATCGCCTGTCG 124,493-124,473 at 1.116
NG2ov-3′r GGAACGCCTTGAAGAGAATGTC 119,613-119,634 at 1.116
nHygB-f CTTGGCTGGAGCTAGTGGAGGT For amplification of hygB cassette from pBCATPH
nHygB-r GGCTGGTGACGGAATTTTCATA For amplification of hygB cassette from pBCATPH
a

Underlined sequences with the same patterns (e.g., light underline, heavy underline, double underline, dotted underline) are complementary and promote hybridization between the PCR products.

b

The positions indicated as bp at contig are from the F. graminearum database at http://www.broad.mit.edu/annotation/fungi/fusarium/index.html.