TABLE 1.
Primers used in this study
| Primer | Sequencea (5′ to 3′) | Positionb (bp at contig) or description |
|---|---|---|
| G2-5′f | TTTGCGGGATGATATGACTGAG | 123,112-123,091 at 1.116 |
| G2-5′r | CTCCACTAGCTCCAGCCAAGC | 121,978-121,999 at 1.116 |
| AAACGCAGCTAACAGAGGAGA | ||
| G2-3′f | TGAAAATTCCGTCACCAGCCTC | 120,415-120,394 at 1.116 |
| TCGCGGCAGCTTGTAATCAT | ||
| G2-3′r | GCCATGCTAGCCCAACTCTCC | 118,949-118,969 at 1.116 |
| NG2-5′f | GCTTGCATCGGACCGAAGGAATGA | 122,880-122,856 at 1.116 |
| NG2-3′r | GCCCTGAGCTTGCAGCAGAGTGTCTT | 119,164-119,189 at 1.116 |
| P12-for | TATAGGGGATTGTGCTTC | 136,498-136,481 at 1.116 |
| P12-rev | AATACACAAACAGCCCTCTC | 129,906-129,925 at 1.116 |
| G1-for | AGGAGCCCTCCAAGTAAT | 144,157-144,140 at 1.116 |
| G1-rev | TCAACGCAGTCGATTGTA | 141,752-141,769 at 1.116 |
| G2-for | ACGACCTTCCTAAACTGCACCTATCA | 122,182-122,157 at 1.116 |
| G2-rev | GGATGCCTGCACCCACCTA | 120,235-120,253 at 1.116 |
| G3-for | GGCTTAACGGCTGAGGACCAAT | 122,940-122,961 at 1.116 |
| G3-rev | GCACGGCCGATCTCATCAAG | 124,669-124,650 at 1.116 |
| G4-for | GCATCACCATTCAATCCT | 127,093-127,076 at 1.116 |
| G4-rev | CCTTTTTATTGCATTGCA | 125,277-125,294 at 1.116 |
| G5-for | TCTCGTCAATACCAACCC | 127,538-127,555 at 1.116 |
| G5-rev | GCAGATTGCCTTCATTCT | 129,844-129,827 at 1.116 |
| G6-for | CGCCTCATAGTGATACCCAAGAAA | 139,925-136,948 at 1.116 |
| G6-rev | AAACGCGGATCTGCCTTCAT | 137,264-137,245 at 1.116 |
| G7-for | GAACACGGCATCCACTGTAAGAT | 139,307-139,285 at 1.116 |
| G7-rev | GCGATATCAGCGAGATCAAAAATA | 137,647-137,670 at 1.116 |
| G8-for | CACCCCGACCCGAAGAGC | 139,452-139,469 at 1.116 |
| G8-rev | CTCACAACAGTCAATCAGGAACCAC | 141,641-141,617 at 1.116 |
| G9-for | TCATCAATGTCAGCCAAG | 144,377-144,394 at 1.116 |
| G9-rev | CGAGTCGCACTGAGTATG | 145,843-145,826 at 1.116 |
| G10-for | GCTTGCCATCTCGAGTTTGAAT | 148,254-148,233 at 1.116 |
| G10-rev | CAGTGCGACTCGAATGAGGC | 145,832-145,851 at 1.116 |
| G2ov-p1 | CATTACATACAGCCGTCAATA | 121,713-121,692 at 1.116 |
| .......................................................... | ||
| TGAGTTCCACAGACCCCCTTC | ||
| G2ov-p2 | GTGTTGGTGCCGTTGCCC | 119,150-119,167 at 1.116 |
| G2ov-p3 | TGTTCGTCGTCGGCTTCGTTC | 123,273-123,293 at 1.116 |
| GCGGGGTCTACAATAAGC | ||
| G2ov-p4 | GCACGGCCGATCTCATCAAG | 124,669-124,650 at 1.116 |
| bpro-5′ | CGAAGCCGACGACGAACA | 49,310-49,327 at 1.393 |
| bpro-3′ | ATTGACGGCTGTAGATGTAATG | 51,455-51,434 at 1.393 |
| ................................................................. | ||
| NG2ov-5′f | ACCCAATACTATCGCCTGTCG | 124,493-124,473 at 1.116 |
| NG2ov-3′r | GGAACGCCTTGAAGAGAATGTC | 119,613-119,634 at 1.116 |
| nHygB-f | CTTGGCTGGAGCTAGTGGAGGT | For amplification of hygB cassette from pBCATPH |
| nHygB-r | GGCTGGTGACGGAATTTTCATA | For amplification of hygB cassette from pBCATPH |
a
Underlined sequences with the same patterns (e.g., light underline, heavy underline, double underline, dotted underline) are complementary and promote hybridization between the PCR products.
b
The positions indicated as bp at contig are from the F. graminearum database at http://www.broad.mit.edu/annotation/fungi/fusarium/index.html.