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Construction of mutants CJM110 and CJM112 via Campbell-type homologous recombination in the small cluster of P. naphthalenivorans strain CJ2 naphthalene degradation genes. (A) The mutant CJM110 was designed to create a polar knock out of regulator nagR2. (B) The mutant CJM112 reported transcriptional activity in the small cluster of the naphthalene degradation pathway.
