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. 2006 Feb;72(2):1373–1379. doi: 10.1128/AEM.72.2.1373-1379.2006

FIG. 1.

FIG. 1.

Neutral lipid biosynthesis in recombinant E. coli BL21(DE3) strains. Cells were cultivated for 40 h at 37°C in LB medium (lanes 1 to 3) or LB medium supplemented with 0.2% (wt/vol) sodium oleate (lanes 4 to 6) and analyzed by TLC. Total lipid extracts obtained from 3 mg lyophilized cells each were applied to lanes 1 to 6. Lanes: A, butyl oleate (40 μg); B, oleyl oleate (40 μg); 1 and 4, E. coli BL21(DE3) (pBBR1MCS-2::atfA); 2 and 5, E. coli BL21(DE3) (pCGN7800); laes 3 and 6, E. coli BL21(DE3) (pCGN7800 plus pBBR1MCS-2::atfA).