FIG. 1.
Growth analysis of Paenibacillus sp. strain JDR-2. (A) Paenibacillus sp. strain JDR-2 growth characterization on individual sugar substrates. OD600 measurements were determined for 4-ml cultures. For these cultures 4 ml of 1% carbohydrate in Z-H mineral salts was inoculated with 200 μl from an overnight culture of 1% YE in Z-H mineral salts. This was started from a single colony of Paenibacillus sp. strain JDR-2 from a 2-day culture in 1% YE with 0.5% oat spelt xylan on Z-H agar plates. (B) Paenibacillus sp. strain JDR-2 growth on 0.5% SG MeGAXn. (C) Paenibacillus sp. strain JDR-2 growth on 0.5% SG MeGAXn and 0.5% glucose. (D) Paenibacillus sp. strain JDR-2 growth on 0.5% SG MeGAXn and 0.5% xylose. (B to D) OD600 measurements of 50-ml cultures in 125-ml baffle flasks. For these 50-ml baffle flask cultures, 4 ml of inoculum was used from an overnight culture in 1% YE in Z-H mineral salts. HPLC was used to quantify carbohydrate concentrations. (A) Square, growth on SG MeGAXn; triangle, growth on xylose; circle, growth on glucose. (B to D) Diamond, OD600; square, SG MeGAXn; triangle, xylose; circle, glucose.