TABLE 2.
Enzyme activities in cell extracts of cultures grown on 3NTyra
| Strain | Enzyme assayed | Assay substrateb | Sp act (nmol min−1 mg protein−1)c |
|---|---|---|---|
| Burkholderia sp. strain JS165 | 3-Nitrotyrosine deaminase | 3NTyr, α-KG | 88 |
| 3-Nitrotyrosine deaminase | 3NTyr, no α-KG | NDd | |
| HNPA denitrase | HNPA, NADH | 40 | |
| HNPA denitrase | HNPA, no added NADH | 14 | |
| HNPA denitrase | HNPA, NAD+ recyclee | ND | |
| 3NTyr denitrase | 3NTyr, NADH | ND | |
| V. paradoxus JS171 | HNPA denitrase | HNPA, NADH | 26 |
| HNPA denitrase | HNPA, no NADH | ND | |
| 3NTyr denitrase | 3NTyr, NADH | ND |
a
No activity was detected in extracts of cells grown on Tyr or succinate.
b
Substrates were provided at a concentration of 200 μM.
c
The values are averages from duplicate reactions.
d
ND, not detected.
e
Preincubation of cell extract with lactate dehydrogenase and pyruvate.