TABLE 2.
Fatty acid compositions of species related to the BCCa
| Fatty acidb or comparison | Fatty acid composition (%)c or species identification of:
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| B. gladioli DSM 4285T | P. norimberg. DSM 11628T | P. apista CCM 4976T | P. pnomenusa CCM 4978T | P. pulmonic. CCM 4979T | P. spurorum CCM 4980T | R. pickettii DSM 6297T | C. necatori | Achromobacter xylosoxidans xylosoxidansi | Delftia acidovoransi | I. limosum DSM 16000T | ||
| Saturated | ||||||||||||
| 12:0 | trd (1) | 2.7 ± 0.2 | 1.8 ± 0.1 | 1.5 ± 0.1 | 2.0 ± 0.1 | 1.7 ± 0.2 | 0.1 (1) | ne | 0.5 | 2.5 | NDf | |
| 14:0 | 3.9 ± 0.2 | 0.3 ± 0.0 | 0.5 ± 0.1 | 0.7 ± 0.0 | 0.4 ± 0.0 | 0.5 ± 0.1 | 4.4 ± 0.2 | 2.8 | 2.1 | 0.7 | ND | |
| 16:0 | 24.9 ± 0.8 | 17.8 ± 0.8 | 24.7 ± 1.3 | 21.0 ± 0.8 | 22.7 ± 0.7 | 21.3 ± 1.3 | 26.7 ± 2.1 | 24.5 | 34.7 | 33.2 | 1.4 | |
| 17:0 | 0.2 ± 0.1 | 0.5 ± 0.1 | 0.2 (1) | 0.3 ± 0.0 | 0.3 ± 0.0 | 0.3 ± 0.0 | 0.5 ± 0.1 | 1.6 | n | n | 4.2 | |
| 18:0 | 1.1 ± 0.2 | 0.9 ± 0.0 | 0.5 ± 0.1 | 0.9 ± 0.1 | 1.0 ± 0.1 | 0.6 ± 0.1 | 0.4 ± 0.0 | 0.4 | 2.6 | n | 1.3 | |
| Unsaturated | ||||||||||||
| 13:1 AT 12-13 | 0.5 ± 0.6 | 0.7 ± 0.6 | 0.6 ± 0.6 | 1.2 ± 1.2 (2) | 0.7 ± 0.7 | 0.3 ± 0.3 | ND | n | n | n | ND | |
| 16:1ω5c | ND | 0.3 ± 0.1 | 0.3 ± 0.1 | 0.2 (1) | 0.3 (1) | 0.4 ± 0.1 | 0.2 ± 0.0 (2) | n | n | n | 0.3 | |
| 16:1ω7cg | 16.0 ± 4.0 | 22.4 ± 3.9 | 23.8 ± 2.1 | 20.2 ± 1.8 | 20.8 ± 2.0 | 13.5 ± 1.4 | 33.7 ± 1.1 | 35.5 | 31.4 | 40.1 | 3.4 | |
| 18:1ω7c | 28.0 ± 2.73 | 33.6 ± 1.6 | 27.6 ± 1.6 | 34.1 ± 1.1 | 34.0 ± 0.8 | 30.8 ± 4.7 | 20.2 ± 1.6 | 22.3 | 9.7 | 17.2 | 46.7 | |
| Hydroxy | ||||||||||||
| 10:0 3OH | ND | ND | ND | ND | ND | ND | ND | n | n | 2.5 | ND | |
| 12:0 2OH | ND | 0.3 ± 0.1 | 0.9 ± 0.0 | 0.8 ± 0.1 | 0.7 ± 0.0 | 1.2 ± 0.1 | ND | n | 2.5 | n | ND | |
| 14:0 2OH | ND | ND | ND | 0.2 (1) | 0.1 (1) | 0.1 ± 0.0 | 0.8 ± 0.3 | 2.5 | 2.2 | n | ND | |
| 14:0 3OHh | 4.5 ± 0.3 | 5.7 ± 0.4 | 5.2 ± 0.0 | 5.0 ± 0.5 | 5.4 ± 0.2 | 5.4 ± 0.0 | 6.4 ± 0.1 | 9.2 | 7.6 | n | 1.2 | |
| 16:0 2OH | 0.9 ± 0.2 | 0.2 ± 0.1 (2) | 0.2 (1) | 0.3 ± 0.1 | 0.2 (1) | 0.5 ± 0.1 | 0.2 (1) | n | n | n | 0.8 | |
| 16:0 3OH | 3.9 ± 0.6 | 4.1 ± 0.5 | 4.4 ± 0.6 | 3.8 ± 1.0 | 4.5 ± 0.5 | 4.5 ± 0.2 | 0.1 (1) | n | n | n | 1.3 | |
| 16:1 2OH | 1.0 ± 0.1 | 0.4 ± 0.1 | 0.5 ± 0.2 | 0.5 ± 0.1 | 0.3 (1) | 0.4 ± 0.2 | 1.2 ± 0.5 | n | n | n | ND | |
| 18:1 2OH | 1.3 ± 0.2 | 0.8 ± 0.2 | 0.8 ± 0.2 | 1.8 ± 0.5 | 0.6 ± 0.1 | 1.9 ± 0.4 | 3.0 ± 0.6 | n | n | n | 15.9 | |
| Cyclo | ||||||||||||
| 17:0 cyclo | 8.5 ± 3.1 | 6.1 ± 3.3 | 6.2 ± 2.0 | 5.6 ± 0.8 | 4.2 ± 1.5 | 10.8 ± 3.0 | 0.8 ± 0.2 | n | 5.6 | 3.3 | ND | |
| 19:0 cyclo ω8c | 5.1 ± 3.1 | 2.7 ± 1.6 | 2.0 ± 1.0 | 2.5 ± 0.3 | 2.1 ± 1.0 | 5.4 ± 2.0 | ND | n | n | n | 9.6 | |
| Library comparison | ||||||||||||
| TSBA50j | B. gladioli/ B. cepacia | B. pyrrocinia/ P. pnomenusa | P. apista | P. pnomenusa | P. pnomenusa/ P. pulmonicola | B. cepacia | R. pickettii/ Erwinia chrysanthemi biotype II | Library match | Library match | Library match | No match | |
Fatty acid compositions of BCC type strains are given in Table 1. Fatty acids present in all species in an amount lower than 0.5% were omitted. Cultivation conditions for the TSBA50 standard library were used.
In a designation, the first value is number of C atoms in the fatty acid. The number following the colon is the number of double-bound C atoms. The value following the letter ω signifies the position in the carbon chain (counted from opposite the carboxyl group). The number preceding OH indicates the number of OH groups. cyclo, cyclopropane circle; c, cis conformation.
Values are means ± standard deviations. Values in parentheses show how many times the fatty acid appeared. Mean values were calculated either for three independent analyses (type strains) or for several isolates (one isolate each for B. multivorans, B. cenocepacia, and B. stabilis) by using FAME data from the standard MIS Sherlock composition report. With the aid of MS Excel software, the standard deviations were calculated according to the formula [sgrt (Σ(x − x′)2/(n − 1))]. Values in boldface type clearly differentiate individual species or genera.
tr, trace amount (fatty acid was present in an amount lower than 0.1%).
n, not mentioned.
ND, not detected.
Detected as Summed feature 3, which can comprise C15:0 iso 2OH, C16:1ω7c, or any combination of these fatty acids. Summed feature 3 probably corresponds to C16:1ω7c, as this fatty acid has been reported for Burkholderia species (2).
Detected as Summed feature 2, which can comprise C14:0 3OH, C16:1 iso I, an unidentified fatty acid with an equivalent chain length value of 10.928, C12:0 ALDE, or any combination of these fatty acids. Summed feature 2 probably corresponds to C14:0 3OH, as this fatty acid has been reported for Burkholderia species (2).
Data were taken from the TSBA50 library of MIS Sherlock. Data for Cupriavidus necator are included in the library as Ralstonia eutropha.
All similarity indexes were calculated as a good library match (0.7 or higher). The similarity index is a software-generated calculation of the distance, in multidimensional space, between the profile of the unknown and the mean profile of the closest library entry. Organisms given could come out as a good library match solely or together as the two closest matches. Apart from individual BCC species, Pandoraea norimbergensis, Pandoraea sputorum, and Inquilinus limosum are not included in the TSBA50 library.