TABLE 6.
Results of M. pneumoniae detection in 2004
| Sample no. and test description | Input (CCU/ 100 μl), pool no.a | Laboratory no. and result
|
% Positive | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 1b | 2 | 3 | 4 | 5 | 6 | 8 | 9 | 11 | 11c | 14 | 16 | 17 | 20 | 18 | 18c | 19c | |||
| 4 | 5,000, p2 | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | 100 |
| 6 | 5,000, p1 | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | − | 94.4 |
| 1 | 500, p1 | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | − | 94.4 |
| 7 | 500, p2 | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | − | − | 88.9 |
| 8 | 5,000, p3 | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | − | − | + | 88.9 |
| 9 | 500, p3 | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | − | − | 88.9 |
| 2 | 0, p3 | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | 0 |
| 3 | 0, p1 | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | 0 |
| 5 | 0, p2 | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | 0 |
| 10 | 0, p1 | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | 0 |
| % Correct results | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 90 | 70 | 60 | ||
| Routine application NAATd | Y | Y | N | Y | N | Y | Y | Y | N | Y | Y | N | Y | N | Y | Y | ? | N | ||
| No. of samples tested monthlye | 3 | 3 | 0 | 1 | 0 | 1 | 2 | 2 | 0 | 3 | ? | 0 | 2 | 0 | 1 | 1 | ? | 0 | ||
a
p1, clear BAL pool; p2, more viscous BAL pool; p3, BAL pool blood stained.
b
Results of multiplex NASBA.
c
Results of multiplex PCR.
d
All operations were performed in separate rooms.
e
0 = 0; 1 = 1 to 10 samples monthly, 2 = 11 to 45; 3 means more than 50.