TABLE 7.
Sample no. and test description | Input (IFU/100 μl) | Laboratory no. and result
|
% Positive | ||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1a | 2 | 6 | 11 | 15 | 3 | 8 | 14 | 4 | 9 | 13 | 15b | 5 | 12 | 10 | |||
B-004 | 4.9 × 102 | + | + | + | + | + | + | + | + | + | NA | + | + | + | + | + | 100 |
B-008 | 4.9 × 102 | + | + | + | + | + | + | + | + | + | NA | + | + | + | + | + | 100 |
A-008 | 4.9 × 101 | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | 100 |
B-005 | 4.9 × 102 | + | + | + | + | + | + | + | + | + | NA | + | + | + | − | + | 93.3 |
A-002 | 4.9 × 101 | + | + | + | + | + | + | + | + | + | + | + | + | + | + | − | 93.8 |
A-006 | 4.9 × 101 | + | + | + | + | + | + | + | + | + | + | + | + | + | + | − | 93.8 |
B-002 | 4.9 × 101 | + | + | + | + | + | + | + | + | + | NA | + | − | + | + | − | 86.7 |
B-003 | 4.9 × 101 | + | + | + | + | + | + | + | + | + | NA | + | − | + | + | − | 86.7 |
B-009 | 4.9 × 101 | + | + | + | + | + | + | + | + | + | NA | + | + | + | − | − | 86.7 |
A-003 | 4.9 | + | + | + | + | + | + | − | + | + | + | + | + | − | − | − | 75.0 |
A-009 | 4.9 | + | + | + | + | + | − | + | + | − | − | − | + | − | + | − | 62.5 |
A-010 | 4.9 | + | + | + | + | + | + | + | − | − | + | − | + | − | − | − | 62.5 |
A-001 | 0 | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | 0 |
A-004 | 0 | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | 0 |
A-005 | 0 | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | 0 |
A-007 | 0 | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | 0 |
B-001 | 0 | − | − | − | − | − | − | − | − | − | NA | − | − | − | − | − | 0 |
B-006 | 0 | − | − | − | − | − | − | − | − | − | NA | − | − | − | − | − | 0 |
B-007 | 0 | − | − | − | − | − | − | − | − | − | NA | − | − | − | − | − | 0 |
B-010 | 0 | − | − | − | − | − | − | − | − | − | NA | − | − | − | − | − | 0 |
% Correct results | 100 | 100 | 100 | 100 | 100 | 95.0 | 95.0 | 95.0 | 90.0 | 90.0 | 90.0 | 90.0 | 85.0 | 80.0 | 60.0 | ||
Routine application NAATc | Y | Y | Y | N | Y | Y | Y | N | N | N | Y | Y | Y | Y | ? | ||
No. of samples tested monthlyd | 3 | 2 | 2 | 0 | 3 | 2 | 1 | 0 | 0 | 0 | 1 | 3 | 1 | 3 | 1 |
Both conventional and real-time NASBA gave the same results.
Results of multiplex NASBA.
All operations were performed in separate rooms.
0 = 0; 1 = 1 to 10 samples monthly; 2 = 11 to 45; 3 = more than 50. NA, technical error.