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Time course of p32, p54, and pp62 recombinant protein expression in insect cells. Extracts from H5 cells infected with Bacp32, Bacp54, and Bacpp62 and collected at 24, 48, 72, or 96 h postinfection, were analyzed by Western blotting (A) or ELISA (B). Wild-type (WT) baculovirus-infected cells were used as negative controls in ELISA tests. ASF-positive reference serum diluted 1:30 (Western blot) or 1:20 (ELISA) was used to reveal the ASFV proteins. Lane M contains molecular mass markers.
