TABLE 1.
Further characterization of Y. enterocolitica strains identified by clinical laboratories
| Strain | Yr | Original IDa (37°C) | API 20E profile (30°C) | STb | Morphologyc | CR-MOXd | Reactions (25°C) in BTe
|
srbf | fuc | YeO:3RS profileg | ID after sequencingh | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| esc | sal | pyz | lip | xyl | tre | ind | VP | BT | |||||||||||
| IH 40555 | 1988 | Y. enterocolitica | 1114523 (99.7%) | NA | Y. mollaretii | − | − | − | + | − | + | + | − | + | NBT | + | − | W | Y. mollaretii |
| IH 41571 | 1995 | Y. enterocolitica? | 1014763 (53%) | NA | Mostly like Y. kristensenii | − | − | − | + | − | + | + | − | (+) | NBT | − | − | ND | Y. rohdei |
| IH 110292 | 1997 | Y. enterocolitica | 1114523 (99.7%) | NT | Y. mollaretii | − | − | − | + | − | + | + | − | − | NBT | + | − | W | Y. mollaretii |
| IH 111322 | 1999 | Y. enterocolitica | 0114523 (99.9%) | NA | Mostly like Y. mollaretii | Ca+ | − | − | + | − | + | + | − | + | NBT | − | + | W | Y. bercovieri |
| IH 111501 | 1999 | Y. enterocolitica | 1114523 (99.7%) | NA | Y. bercovieri | − | − | − | + | − | + | + | + | + | NBT | − | + | W | Y. bercovieri |
| IH 111541 | 1999 | Y. enterocolitica | 1014522 (93.9%) | NA | Mostly like Y. mollaretii | (+) | − | − | (+) | − | + | + | − | + | NBT | + | − | W | Y. mollaretii |
| IH 116003 | 2000 | Y. enterocolitica? | 1114703 (26.2%) | NA | Unique | − | − | − | + | − | + | + | − | (+) | NBT | + | − | W | Y. bercovieri |
| IH 116025 | 2000 | Y. enterocolitica | 0114523 (99.9%) | NA | Mostly like Y. bercovieri | − | − | − | + | − | + | + | − | − | NBT | − | + | W | Y. bercovieri |
| IH 116028 | 2000 | Y. enterocolitica | 0014523 (97.7%) | NA | Mostly like Y. bercovieri | − | − | − | + | − | + | + | − | − | NBT | − | + | W | Y. bercovieri |
| IH 111767 | 2000 | Y. enterocolitica | 1014522 (93.9%) | NT | Mostly like Y. mollaretii | − | − | − | − | − | + | + | − | − | BT3 | + | − | W-I | Y. mollaretii |
| IH 111778 | 2000 | Y. enterocolitica | 0154723 (99.2%) | NT | Atypical Y. enterocolitica BT1A | − | + | + | + | + | + | + | − | − | NBT | + | + | W | Y. enterocolitica |
| IH 111799 | 2000 | Y. enterocolitica? | 1014122 (13.8%) | NT | Y. mollaretii | Ca+ | − | − | (+) | − | + | + | − | + | NBT | + | − | W | Y. mollaretii |
| Comparison strains | |||||||||||||||||||
| IH 111430 | 1999 | Y. enterocolitica | 1154763 (97.8%) | NT | Mostly like Y. enterocolitica | (+) | + | + | + | + | + | + | + | + | BT1A | ND | ND | W | Y. enterocolitica |
| IH 111517 | 1999 | Y. enterocolitica | 1014523 (96.4%) | O:3 | Y. enterocolitica O:3 | + | − | − | − | − | − | + | − | − | BT 4 | ND | ND | 3.2a | Y. enterocolitica |
| IH 116007 | 2000 | Y. enterocolitica | 0114723 (99.9%) | O:3 | Y. enterocolitica O:3 | + | − | − | − | − | − | + | − | − | BT 4 | ND | ND | 3.11k | Y. enterocolitica |
| IH 41122 | 1991 | Y. enterocolitica | 1154523 (92.3%) | O:9 | Y. enterocolitica O:9 | + | − | − | − | − | + | + | − | + | BT 3 | ND | ND | 9.4b | Y. enterocolitica |
| IH 41334 | 1993 | Y. enterocolitica | 1015723 (92.8%) | O:5 | Y. enterocolitica O:5,27 | + | − | − | − | − | + | + | − | + | BT 3 | ND | ND | 5.6b | Y. enterocolitica |
Identified by different hospital laboratories.
Serotype of the strain determined by agglutination with sera O:3, O:5, O:8, and O:9 by Denka Seiken (Tokyo, Japan). NA, not agglutinating with the sera used; NT, not typeable, cross-reacting with the sera used.
Colony morphology on CIN agar (incubation at 30°C for 22 to 24 h) observed through a stereomicroscope and compared to ATCC reference strains.
Congo red magnesium oxalate agar test (13) for the presence of the virulence plasmid at 37°C for 24 and 48 h. −, negative; (+), weak positive; +, positive; Ca+, strain has pinpoint colonies showing calcium dependence (5) but does not bind Congo red.
Y. enterocolitica strains divided into BT 1 to BT 5 according to Wauters et al. (18). BT consists of tests for esculin (esc), salicin (sal), pyrazinamidase (pyz), lipase (Tween-esterase) (lip), xylose (xyl), trehalose (tre), indole (ind), and Voges-Proskauer at 25°C for 24 and 48 h. NBT, not biotypeable.
Srb, sorbose; fuc, fucose tests at 25°C for 24 and 48 h. ND, not determined.
Genotyping using Y. enterocolitica O:3 repeated sequence probe (6). W, weak hybridization; I, incomplete typing pattern; ND, not determined.