TABLE 1.
Primers used in this studya
| Gene | Primer | Nucleotide sequence | Cycling conditionsb |
|---|---|---|---|
| sdrC | sdrCF | 5′-AAAAGGCATGATACCAAATCGA | Den, 94°C/30 s; Ann, 53°C/30 s; Elon, 72°C/15 s |
| sdrCR | 5′-AATTCTCCATTCGTATGTTCTG | ||
| sdrD | sdrDF | 5′-AGTGGGAACAGCATCAATTTTA | |
| sdrDR | 5′-GTGGTAGATTGTACACTTTCTT | ||
| sdrE | sdrEF | 5′-AGAAAGTATACTGTAGGAACTG | |
| sdrER | 5′-GATGGTTTTGTAGTTACATCGT | ||
| Azoreductase | azoF | 5′-GTGAATAATAG(C/T)CCATATGAAC | Den, 94°C/30 s; Ann, 50°C/30 s; |
| Putative glycosyltransferase | glyR | 5′-CTACACCTGTAATTTTACTTTC | Elon, 72°C/1 min |
a
The primers were designed based on S. aureus genomes available from the Institute for Genomic Research (http://www.tigr.org) (COL strain), the University of Oklahoma (http://www.genome.ou.edu) (strain 8325), the Sanger Institute (http://www.sanger.ac.uk) (strains MRSA252 and MSSA476), the National Institute of Technology and Evaluation (http://www.bio.nite.go.jp) (strain MW2), and Juntendo University (http://www.juntendo.ac.jp) (strains Mu50 and N315).
b
Den, denaturation temperature and time; Ann, annealing temperature and time; Elon, elongation temperature and time.