TABLE 1.
Detection of C. trachomatis diluted in UTM from FS and KS processed in AC2, AMP, and PT assaysa
| Dilutions of C. trachomatis in UTM (10 tubes/dilution) | No. of positive results (n = 40) obtained by NAAT:
|
Culture in McCoy shell vial cultures (fluorescent inclusions in duplicate/ total no.) | |||||
|---|---|---|---|---|---|---|---|
| AC2
|
AMP
|
PT
|
|||||
| KS | FS | KS | FS | KS | FS | ||
| 10−5 | 10 | 10 | 10 | 10 | 10 | 10 | 43/40 |
| 10−6 | 10 | 10 | 10 | 10 | 10 | 10 | 10/6 |
| 10−7 | 10 | 10 | 7 | 10 | 1 | 3 | 0 |
| 10−8 | 2 | 8 | 0 | 1 | 0 | 0 | 0 |
| 10−9 | 1 | 4 | 0 | 0 | 0 | 0 | 0 |
| 10−10 | 0 | 3 | 0 | 0 | 0 | 0 | 0 |
a
Each dilution was used to inoculate 10 replicates for each swab type, resulting in a maximum of 10 positive results for each dilution-swab combination in each assay. The numbers of positive results (%) per 40 samples that were below the 100% endpoint (below 10−6) were as follows: AC2 KS, 13 (32.5%); AC2 FS, 25 (62.5%); AMP KS, 7 (17.5%); AMP FS, 11 (27.5%); PT KS, 1 (2.5%); and PT FS, 3 (7.5%). P values for these results were as follows: AC2, P < 0.001; AMP, P = 0.13; PT, P = 1.0; AC2 versus AMP, P = 0.3; AC2 versus PT, P < 0.001.