TABLE 2.
Detection of N. gonorrhoeae diluted in UTM from FS and KS processed in AC2, AMP, and PT assaysa
| Dilutions of N. gonorrhoeae in UTM (10 tubes/dilution) | No. of positive results (n = 30) obtained by NAAT:
|
Culture in chocolate agar (no. of colonies/ 0.010 ml in duplicate) | |||||
|---|---|---|---|---|---|---|---|
| AC2
|
AMP
|
PT
|
|||||
| KS | FS | KS | FS | KS | FS | ||
| 10−4 | 10 | 10 | 10 | 10 | 10 | 10 | >1,000 |
| 10−5 | 10 | 10 | 10 | 10 | 10 | 10 | 48/42 |
| 10−6 | 10 | 10 | 4 | 6 | 6 | 8 | 7/9 |
| 10−7 | 7 | 9 | 0 | 2 | 1 | 3 | 0 |
| 10−8 | 0 | 3 | 0 | 0 | 0 | 0 | 0 |
a
Each dilution was used to inoculate 10 replicates for each swab type, resulting in a maximum of 10 positive results for each dilution-swab combination in each assay. The numbers of positive results (%) per 30 samples that were below the 100% endpoint (below 10−5) were as follows: AC2 KS, 17 (56.6%); AC2 FS, 22 (73.3%); AMP KS, 4 (13.3%); AMP FS, 8 (26.6%); PT KS, 7 (23.3%); and PT FS, 11 (36.6%). P values for these results were as follows: AC2, P = 0.06; AMP, P = 0.13; PT, P = 0.13; AC2 versus AMP, P < 0.001; AC2 versus PT, P = 0.002.