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MaeI digestion of PCR product amplified from domain V of the 23S rRNA gene of E. faecium and E. faecalis for presence of the G2576T mutation. Positive control strains are shown in lanes 1 to 4. Lanes 5 to10 show linezolid susceptible (lane 5), intermediate (lanes 6, 8, and 9), and resistant (lanes 7 and 10) clinical strains determined by manual agar dilution and MicroScan broth microdilution.
