TABLE 4.
Clonal complex distribution among invasive pneumococcal isolates in 1999, 2001, and 2002
| Serotype (clonal complex)a | No. of isolates within complex/no. of isolates analyzed (%) in the following years for the indicated age groups
|
|||||
|---|---|---|---|---|---|---|
| 1999
|
2001
|
2002b
|
||||
| <5 yr | ≥5 yr | <5 yr | ≥5 yr | <5 yr | ≥5 yr | |
| 1 (ST227) | 5/6 (83.3) | 15/15 (100) | 4/4 (100) | 28/34 (82.4) | 4/4 (100) | None analyzed |
| 1 (ST217) | 1/6 (16.7) | 0/15 | 0/4 | 1/34 (2.9) | 0/4 | None analyzed |
| 1 (ST1174) | 0/6 | 0/6 | 0/6 | 2/34 (5.9) | 0/4 | None analyzed |
| 3 (ST180) | 6/6 (100) | 48/50 (96.0) | 6/6 (100) | 68/70 (97.0) | 8/8 (100) | 11/11 (100) |
| 4 (CC899) | 53/71 (74.6) | None analyzed | 29/35 (82.9) | 18/34 (52.9) | None analyzed | 1/1 (100) |
| 4 (ST205) | 8/71 (11.3) | None analyzed | 6/35 (17.1) | 11/34 (32.4) | None analyzed | 0/1 (0) |
| 5 (ST653) | 100 (1) | None analyzed | 1/1 (100) | 1/1 (100) | No type 5s | None analyzed |
| 6A (CC1296) | 19/57 (33.3) | 0/1 | 10/38 (26.3) | 0/9 | 4/13 (30.7) | 14/41 (34.1) |
| 6A (CC460) | 13/57 (22.4) | 0/1 | 11/38 (29.4) | 3/9 (33.3) | 3/13 (23.1) | 0/41 |
| 6A (CC473) | 5/57 (8.8) | 0/1 | 7/38 (18.4) | 1/9 (11.1) | 1/13 (7.7) | 15/41 (36.6) |
| 6A (ST395) | 1/57 (1.8) | 1/1 (100) | 3/38 (7.9) | 0/9 | 3/13 (23.1) | 2/41 (4.9) |
| 6A (CC490) | None detectedb | 0/1 | 2/38 (5.3) | 0/9 | None detected | None detected |
| 6A (CC138) | None detected | 0/1 | 2/38 (5.3) | 0/9 | None detected | None detected |
| 6A (ST1091) | None detected | 0/1 | 1/38 (2.6) | 0/9 | None detected | None detected |
| 6A (ST1175) | None detected | 0/1 | 1/38 (2.6) | 0/9 | None detected | None detected |
| 6A (ST90) | 1/57 (1.8) | 0 | None detected | 0/9 | None detected | None detected |
| 6A (ST660) | 1/57 (1.8) | 0 | None detected | 0/9 | None detected | None detected |
| 6A (ST690) | None detected | 0 | None detected | 2/9 (22.2) | None detected | None detected |
| 6A (CC1292) | None detected | 0/1 | None detected | 3/9 (33.3) | None detected | 6/41 |
| 6B (CC138) | 13/103 (12.6) | 0/1 | 13/45 (28.9) | 2/15 (9.1) | None analyzed | 1/4 (25.0) |
| 6B (CC146) | 22/103 (21.4) | 0/1 | 11/45 (24.4) | 4/15 (28.6) | None analyzed | 0/4 |
| 6B (ST90) | 13/103 (12.6) | 0/1 | 13/45 (28.9) | 5/15 (28.6) | None analyzed | 0/4 |
| 6B (CC384) | 6/103 (5.8) | 0/1 | 5/45 (11.1) | 3/15 (20.0) | None analyzed | 0/4 |
| 6B (CC1269) | 3/103 (2.9) | 0/1 | None detected | 0/15 | None analyzed | 0/4 |
| 6B (CC490) | None detected | 0/1 | 2/45 (4.4) | 0/15 | None analyzed | 3/4 (75.0) |
| 6B (ST185) | 1/103 (1.0) | 0/1 | None detected | 0/15 | None analyzed | 0/4 |
| 6B (ST690) | None detected | 1/1 | None detected | 1/15 (6.7) | None analyzed | 0/4 |
| 7B (ST664) | 1/1 (100) | No type 7Bs | No type 7Bs | No type 7Bs | No type 7B | No type 7Bs |
| 7C (ST1207) | No type 7C | No type 7Cs | No type 7Cs | 1/1 (100) | 1/1 (100) | 1/1 (100) |
| 7F (ST191) | 7/7 (100) | 22/24 (91.7) | 13/15 (86.7) | 29/30 (96.7) | 5/6 (83.3) | 3/3 (100) |
| 7F (CC218) | 0/7 | 1/24 (4.2) | None detected | None detected | None detected | 0/3 |
| 8 (ST53) | No type 8 | None analyzed | 2/2 (100) | 1/10 (10.0) | No type 8 | 100 (1) |
| 8 (ST1268) | No type 8 | None analyzed | 0/2 | 9/10 (90.0) | No type 8 | 0/1 |
| 9A (CC156) | No type 9A | No type 9As | 3/3 (100) | No type 9As | No type 9A | No type 9As |
| 9N (CC66) | 2/2 (100) | 9/11 (81.8) | 3/3 (100) | 34/37 (91.9) | None analyzed | 3/5 (60.0) |
| 9N (CC156) | 0/2 | None detected | 0/3 | None detected | None analyzed | 1/5 (20.0) |
| 9N (CC1257) | 0/2 | None detected | 0/3 | None detected | None analyzed | 1/5 (20.0) |
| 9V (CC156) | 60/72 (83.3) | 21/26 (80.1) | 26/38 (68.4) | 28/42 (66.7) | 2/2 (100) | 7/7 (100) |
| 9V (CC1269) | 9/72 (12.5) | 3/26 (11.5) | 11/38 (28.9) | 14/42 (33.3) | 0/2 | 0/2 |
| 10A (CC460) | 1/1 (100) | None analyzed | 3/3 (100) | 2/5 (40) | 3/3 (100) | 2/2 (100) |
| 10A (ST1263) | 0/1 | None analyzed | 0/3 | 2/5 (40) | 0/3 | 0/2 |
| 11A (CC62) | 1/1 (100) | 22/24 (87.5) | 5/5 (100) | 41/41 (100) | 2/2 (100) | 9/9 (100) |
| 12F (CC218) | 11/11 (100) | 48/49 (97.8) | 17/17 (100) | 65/65 (100) | 4/6 (66.7) | 1/1 (100) |
| 12F (ST989) | 0/11 | None detected | 0/17 | 0/65 | 1/6 (16.7) | 0/1 |
| 12F (CC1292) | 0/11 | None detected | 0/17 | 0/65 | 1/6 (16.7) | 0/1 |
| 13 (CC392) | No type 13 | None analyzed | No type 13s | 2/2 (100) | No type 13 | 0/1 |
| 13 (ST1260) | No type 13 | None analyzed | No type 13s | 0/2 | No type 13 | 1/1 (100) |
| 14 (CC13) | 96/208 (46.2) | None analyzed | 48/91 (52.7) | 19/36 (52.7) | None analyzed | 3/3 (100) |
| 14 (CC124) | 78/208 (37.5) | None analyzed | 33/91 (30.0) | 9/36 (25.0) | None analyzed | 0/3 |
| 14 (CC63) | None detected | None detected | 6/91 (6.6) | 0/36 | None analyzed | 0/3 |
| 14 (CC1269) | 5/208 (2.4) | None analyzed | 2/91 (2.2) | 2/36 (5.9) | None analyzed | 0/3 |
| 14 (CC156) | None detected | None analyzed | 3/91 (3.3) | 1/36 (2.8) | None analyzed | 0/3 |
| 14 (CC66) | 4/208 (1.9) | None analyzed | 1/91 (1.1) | 1/36 (2.8) | None analyzed | 0/3 |
| 15A (CC63) | No type 15As | None analyzed | No type 15As | 3/3 (100) | No type 15As | 12/13 (92.3) |
| 15A (ST817) | No type 15As | None analyzed | No type 15As | 0/3 | No type 15As | 1/13 (7.7) |
| 15BC (CC199) | 6/6 (100) | None analyzed | 20/22 (90.9) | 3/3 (100) | 27/33 (81.8) | 5/5 (100) |
| 15BC (ST1262) | 0/6 | None analyzed | None detected | 0/3 | 2/33 (6.1) | 0/5 |
| 15BC (ST1267) | 0/6 | None analyzed | None detected | 0/3 | 2/33 (6.1) | 0/5 |
| 15BC (ST1259) | 0/6 | None analyzed | None detected | 0/3 | 1/33 (3.0) | 0/5 |
| 15BC (CC156) | 0/6 | None analyzed | None detected | 0/3 | 1/33 (3.0) | 0/5 |
| 16F (CC659) | 1/1 (100) | 0/2 | No type 16Fs | 7/7 (100) | 2/2 (100) | 1/3 (33.3) |
| 16F (CC30) | 0/1 | 2/2 (100) | No type 16s | 0/7 | 0/2 | 2/3 (66.7) |
| 17F (CC392) | No type 17F | None analyzed | 1/1 (100) | None analyzed | 1/1 (100) | None analyzed |
| 18BCF (CC113) | 74/93 (79.6) | None analyzed | 28/40 (68.3) | 9/11 (81.8) | 2/3 (66.7) | 2/2 (100) |
| 18BCF (ST496) | 8/93 (8.6) | None analyzed | 5/40 (12.5) | 1/11 (9.0) | 0/3 | 0/2 |
| 18BCF (ST666) | 1/93 (1.1) | None analyzed | 1/40 (2.4) | 1/11 (9.0) | 1/3 (33.3) | 0/2 |
| 18BCF (CC66) | 1/93 (1.1) | None analyzed | None detected | 0/11 | 0/2 | 0/2 |
| 19A (ST199) | 13/18 (72.2) | 46/64 (71.9) | 18/29 (62.1) | 10/10 (100) | 27/40 (67.5) | 32/45 (71.1) |
| 19A (CC172) | None detected | 2/64 (3.1) | 2/29 (6.9) | 0/10 | 0/40 | 1/45 (2.2) |
| 19A (ST665) | 1/18 (5.5) | None detected | 1/29 (3.4) | None detected | 0/40 | 0/45 |
| 19A (CC236) | None detected | None detected | 1/29 (3.4) | None detected | 3/40 (7.5) | 1/45 (2.2) |
| 19A (CC1296) | None detected | None detected | None detected | None detected | 4/40 (10.0) | 5/45 (11.1) |
| 19A (CC81) | 1/18 (5.5) | None detected | None detected | None detected | 1/40 (2.5) | 2/45 (4.4) |
| 19A (ST230) | None detected | None detected | 2/29 (6.9) | None detected | 0/40 | 0/45 |
| 19A (ST1297) | None detected | None detected | 1/29 (3.4) | None detected | 3/40 (7.5) | 2/45 (4.4) |
| 19A (CC62) | None detected | None detected | None detected | None detected | 2/40 (5.0) | 0/45 |
| 19A (ST1374) | None detected | None detected | None detected | 0/10 | 0/40 | 1/45 (2.2) |
| 19A (ST847) | None detected | None detected | None detected | 0/10 | 0/40 | 1/45 (2.2) |
| 19F (CC236) | 23/110 (20.9) | None examined | 10/47 (21.3) | 3/12 (25.0) | 1/2 (50) | 8/9 (88.9) |
| 19F (CC251) | 22/110 (20.0) | None examined | 19/47 (40.4) | 3/12 (25.0) | 0/2 | 0/9 |
| 19F (CC81) | 13/110 (11.8) | None examined | 3/47 (6.4) | 3/12 (25.0) | 0/2 | 0/9 |
| 19F (CC199) | 10/110 (9.1) | None examined | 1/47 (2.1) | 1/12 (9.1) | 0/2 | 0/9 |
| 19F (ST655) | 2/110 (1.8) | None examined | 0/47 | 0/12 | 0/2 | 0/9 |
| 19F (CC177) | 2/110 (1.8) | None examined | 4/47 (8.5) | 0/12 | 0/2 | 0/9 |
| 19F (CC1269) | 1/110 (0.9) | None examined | 2/47 (4.3) | 1/12 (9.1) | 0/2 | 0/9 |
| 19F (ST1340) | None detected | None examined | 0/47 | 0/12 | 1/2 (50) | 0/9 |
| 19F (CC13) | 1/110 (0.9) | None examined | 0/47 | 0/12 | 0/2 | 0/9 |
| 19F (ST43) | 1/110 (0.9) | None examined | 4/47 (8.5) | 0/12 | 0/2 | 0/9 |
| 19F (CC172) | None detected | None examined | 1/47 (2.1) | 0/12 | 0/2 | 0/9 |
| 19F (CC425) | 4/110 (3.6) | None examined | 3/47 (6.4) | 0/12 | 0/2 | 0/9 |
| 19F (CC63) | None detected | None examined | 0/47 | 1/12 (9.1) | 0/2 | 0/9 |
| 19F (ST1203) | None detected | None examined | 0/47 | 0/12 | 0/2 | 1/9 (11.1) |
| 20 (CC899) | No type 20 | None examined | 1/1 (100) | 4/6 (66.7) | No type 20 | None examined |
| 20 (CC1257) | No type 20 | None examined | 0/1 | 33.3 (2) | No type 20 | None examined |
| 22F (CC433) | 6/6 (100) | 30/31 (96.8) | 19/19 (100) | 90.2 (46) | 92.9 (13) | 100 (2) |
| 22F (ST1294) | 0/2 | None detected | 0/1 | None detected | 7.1 (1) | 0/2 |
| 23A (CC172) | 2/2 (100) | None examined | 0/1 | 0/1 | No type 23A | 5/6 (83.3) |
| 23A (ST1336) | 0/2 | None examined | 1/1 (100) | 0/1 | No type 23A | 0/6 |
| 23A (ST1338) | 0/2 | None examined | 0 | 2/4 (50.0) | No type 23A | 0/6 |
| 23A (CC37) | 0/2 | None examined | 0 | 2/4 (50.0) | No type 23A | 1/6 (16.7) |
| 23F (CC37) | 46/64 (71.9) | None examined | 26/37 (70.3) | 6/13 (46.2) | None examined | 0/5 |
| 23F (CC81) | 11/64 (17.2) | None examined | 5/37 (13.5) | 4/13 (30.7) | None examined | 4/5 (80.0) |
| 23F (ST242) | 4/64 (6.2) | None examined | 1/37 (2.7) | 2/13 (15.4) | None examined | 1/5 (20.0) |
| 23F (CC662) | 1/64 (1.6) | None examined | None detected | 0/13 | None examined | 0/5 |
| 23F (CC268) | None detected | None examined | 1/37 (2.7) | 1/13 (7.7) | None examined | 0/5 |
| 23F (CC460) | None detected | None examined | 2/37 (5.4) | 0/13 | None examined | 0/5 |
| 24BF (CC72) | No type 24BF | None examined | 1/1 (100) | None examined | None examined | 1/1 (100) |
| 28F (ST546) | No type 28F | No type 28F | No type 28F | No 28F isolates | No 28F isolates | 100 (1) |
| 31 (ST568) | No type 31 | 1/1 (100) | No type 31 | 2/2 (100) | 1/1 (100) | No type 31 |
| 33F (CC662) | 7/7 (100) | 1/1 (100) | 10/11 (90.9) | 7/8 (87.5) | 18/18 (100) | 1/1 (100) |
| 33F (ST1012) | 0/7 | 0/1 | 1/11 (9.1) | 1/8 (12.5) | 0/18 | 0/1 |
| 35B (CC377) | 2/2 (100) | 10/14 (71.4) | 2/2 (100) | 27/35 (77.1) | 100 (1) | 18/19 (94.7) |
| 35B (ST452) | 0/2 | 4/14 (28.6) | 0/2 | 8/35 (22.9) | 0/1 | 1/19 (5.3) |
| 35F (ST498) | No type 35F | None examined | 2/2 (100) | 2/2 (100) | None examined | None examined |
| 37 (ST447) | No type 37 | None examined | No type 37 | None examined | No type 37 | 1/2 (50.0) |
| 38 (ST393) | 5/5 (100) | None examined | 7/7 (100) | 1/1 (100) | 15/15 (100) | None examined |
a
Clonal complexes in boldface were found only among isolates from individuals ≥5 years of age. Up to 30% of the isolates within a given serotype had unique PFGE profiles and were not subjected to MLST (represented by gaps in the columns in Fig. 2). Such isolates could possibly belong to any of the clonal sets included or could represent additional clonal sets.
b
Year 2002 isolates were primarily selected from non-PCV7 serotypes.